中国免疫学杂志Issue(12)：1686-1691,6.DOI:10.3969/j.issn.1000-484X.2014.12.023
6种miRNAs在类风湿关节炎患者外周血及关节液中的表达分析
Analysis on expression of six kinds of miRNAs in peripheral blood and synovial fluid for patients with rheumatoid arthritis
摘要
Abstract
Objective:To investigate the expression of miR-16,miR-17,miR-30a etc in peripheral blood plasma ,mononuclear cells ( PBMC) and synovial fluid of patients with rheumatoid arthritis ( RA) and its clinical significance ,to provide a theoretical basis for the further research in the mechanism of RA.Methods:Collected 80 cases of RA patients ,32 cases of osteoarthritis ( OA) patients and 32 healthy human peripheral blood ,synovial fluid ,separating the plasma and PBMC;extraction of small RNA ,with specific stem loop primed reverse transcription into cDNA , establishing a mature miRNA-T carrier and making standard curve;stem-loop method Real-time quantitative PCR was adopted to detect the expression of miRNAs in plasma ,PBMC and synovial fluid,and for correlation analysis;and with RA activity monitoring indicators rheumatoid factor (RF),erythrocyte sedimentation rate (ESR),C-reactive protein (CRP) correlation analysis.Results:In RA group,the expression of miR-16,miR-17,miR-30a,miR-106,miR-101 and miR-130 in plasma,PBMC and synovial fluid were upregμlation compared with OA group,the healthy control group (P<0.05),but the expression level of miR-101 in plasma of RA and OA -group difference was not statistically significant.Correlation analysis showed that 6 kinds of miRNA in the plasma ,PBMC and joint fluid have varying degrees of positive correlation ( P<0.05 ).Correlation analysis showed that miRNA in plasma , PBMC and synovial fluid have one or more indicators of a positive correlation with RF , ESR, CRP ( P<0.05 ).Conclusion:The expression of miR-16,miR-17,miR-30a,miR-101,miR-106 and miR-130 is changes significant in the peripheral blood and synovial fluid of RA patients ,indicate the miRNAs may be through some related genes play an important role in the process of the pathogenesis of RA;its expression level can be used as an effective indicator of RA disease activity , and provide new diagnostic markers for RA.关键词
miRNA/类风湿关节炎/Real-time PCRKey words
microRNA/Rheumatoid arthritis/Real-time PCR分类
医药卫生引用本文复制引用
郑锡铭,刘鑫,周林林,张涛,张爱君,徐广贤..6种miRNAs在类风湿关节炎患者外周血及关节液中的表达分析[J].中国免疫学杂志,2014,(12):1686-1691,6.基金项目
本文为宁夏自然科学基金项目（ NZ14095）、教育部＂新世纪优秀人才支持计划＂项目（ No.NCET11－11023）和国家自然科学基金项目（ No.31160794）。 （）
