中国免疫学杂志Issue(1)：16-21,6.DOI:10.3969/j.issn.1000-484X.2015.01.003
H7 N9流感病毒HA、NA蛋白的抗原表位预测及其与HLA－Ⅱ类等位基因的相关性分析
Prediction of antigenic epitopes on HA, NA amino acid sequences of novel influenza A (H7N9) virus and analysis association between susceptibility and HLA-Ⅱalleles
摘要
Abstract
Objective:To compare the amino acid sequences difference of HA,NA novel influenza virus A/H7N9 isolates, decipher possible B cell epitopes and T cell epitopes of HA,NA protein,and analyze the association between susceptibility and HLA polymorphisms.Methods:The amino acid sequences of novel influenza A ( H7N9) virus were downloaded from Genbank.Phylogenetic trees were constructed based on the amino acid sequences of HA and NA by using software Clustal X and MEGA 4.0.B cell and T cell epitopes were respectively predicted with Protean software and NetMHCⅡ2.2 Server online server.Results:The homology of HA and NA proteins of H7N9 virus was high.10 B cell epitopes and 15 T cell epitopes were randomly distributed throughout HA sequence and 12 B cell epitopes and 9 T cell epitopes were randomly distributed throughout NA sequence.HLA-DRB1*0701 allele which was commonly observed in Northern Chinese population have a high binding affinity for 9-mer peptides of HA and NA proteins.Conclusion:The prediction of B and T cell epitopes of HA and NA proteins with multiple methods benefits the research and development of vaccine against human infection with avian influenza A H7N9 virus.HLA-DRB1*0701 allele may contribute to susceptibility to novel influenza A (H7N9) virus.H7N9 influenza virus is more easily spread in Urumqi,Harbin,Shandong Province,Liaoning Province,Beijing, Shijiazhuang and Tianjin of China.关键词
H7N9流感病毒/抗原性表位/HLA/易感性/血凝素/神经氨酸酶Key words
Influenza virus A/H7N9/Antigenic epitope/HLA/Susceptibility/Hemagglutinin ( HA)/Neuraminidase (NA)分类
医药卫生引用本文复制引用
刘雪婷,王珊,张俊艳,刘兆宇,陈惠芳,邹泽红,肖兰艳,及志恒,何颖..H7 N9流感病毒HA、NA蛋白的抗原表位预测及其与HLA－Ⅱ类等位基因的相关性分析[J].中国免疫学杂志,2015,(1):16-21,6.基金项目
本文受国家自然基金青年项目（81301948）、广东高校优秀青年创新人才培养计划项目（20131 LYM＿0070）、广州医科大学博士启动项目（2012C40）的资助。 （）
