中国免疫学杂志Issue(1)：31-35,39,6.DOI:10.3969/j.issn.1000-484X.2015.01.006
microRNA－27a 对树突状细胞表型及功能的影响
microRNA-27a regulates maturation process and cytokine in LPS-stimulated dendritic cells
摘要
Abstract
Objective:To explore the effects of miR-27a on the phenotype and cytokine secretion in LPS-stimulated dendritic cells.Methods:Murine bone marrow-derived dendritic cells were transfected with miR-27a mimics and negative control mimics,and then stimulated by LPS for 24 hours.Dendritic cells exposed to LPS were collected for analysis of the DC immunophenotype by flow cy-tometry and supernatants were collected to determine cytokine lever.Moreover,the capability of stimulating allogeneic CD4 T+cell prolif -eration was measured by MLR ( mixed lymphocyte reaction) .Results: The levels of MHCⅡ, CD80, and CD86 were significantly increased in LPS-stimulated dendritic cells when compared with imDC ( P<0.001).Transfection with miR-27a mimics resulted in sig-nificantly lower expression levels in levels of MHCⅡ,CD80,and CD86 (P<0.001).For cytokine secretion,transfection with miR-27a mimics enhanced IL-10 production (P<0.01) and reduced the production of IL-12 (P<0.05).For MLR,transfection with miR-27a mimics suppressed allogeneic CD4+T cell proliferation.Conclusion: MiR-27a may play critical roles in regulating the maturation process and cytokine secretion in LPS-stimulated dendritic cells.关键词
MicroRNA-27a/骨髓来源树突状细胞/成熟/细胞因子Key words
microRNA-27a/Bone marrow-derived dendritic cell/Maturation/Cytokine分类
医药卫生引用本文复制引用
刘信攸,周阳春,王瑶,杨晓俊,沈历宗,吴文溪..microRNA－27a 对树突状细胞表型及功能的影响[J].中国免疫学杂志,2015,(1):31-35,39,6.基金项目
本文为国家自然基金资助项目（81070381）。 （）
