中国免疫学杂志Issue(1)：48-51,4.DOI:10.3969/j.issn.1000-484X.2015.01.010
膜表达型InsB15－23 H－2 Kd dtSCT对NOD 小鼠1型糖尿病发病的影响
Membrane-expressed InsB15-23 H-2Kd dtSCT can reduce morbidity of type 1 diabetes mellitus in NOD mice
潘兴元 1陈则东 1窦延 1梁锴 1余鸣鸣 1季明春1
作者信息
- 1. 扬州大学医学院病原生物学与免疫学教研室，扬州 225001
- 折叠
摘要
Abstract
Objective:To illuminate the influence of InsB15-23 H-2Kd dtSCT to the morbidity of type 1 diabetes mellitus in NOD mice.Methods:An eukaryotic plasmid encoded membrane-expressed InsB15-23 H-2Kd dtSCT was inoculated into 3 weeks old female NOD mice subcutaneously and the blood sugar and morbidity of type 1 diabetes mellitus were monitored once a week.To illuminate the cellular mechanism of immunologic intervention of membrane-expressed InsB15-23 H-2Kd dtSCT to the course of type 1 diabetes mellitus in NOD mice,the mononuclear cell infiltration of islets was detected by tissue slice and the frequency of IGRP206 2-14 specific CTLs in PBMC was analyzed by FACs.Results: As compared with pcDNA3.1 (-) control ( 60%) and untreated NOD mice ( 80%) , mice immunized with InsB15-23 H-2Kd dtSCT exhibited low level of islet infiltration and low morbidity in 30 weeks old ( 9%) .But the frequency of IGRP206-214 specific CTLs in PBMC of 16 and 40 weeks old mice showed no difference.Conclusion:Membrane-expressed InsB15-23 H-2Kd dtSCT can protect NOD mice from type 1 diabetes mellitus in IGRP206-214 independent pattern.关键词
二硫键捕获型单链三聚体/CD8+T细胞/1型糖尿病/NOD小鼠Key words
Disulfide-trap single-chain trimer/CD8+T cells/Type 1 diabetes mellitus/NOD mice分类
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潘兴元,陈则东,窦延,梁锴,余鸣鸣,季明春..膜表达型InsB15－23 H－2 Kd dtSCT对NOD 小鼠1型糖尿病发病的影响[J].中国免疫学杂志,2015,(1):48-51,4.
