中国免疫学杂志Issue(1)：86-89,4.DOI:10.3969/j.issn.1000-484X.2015.01.018
人外周血巨噬细胞培养及功能鉴定
Culture and functional identification of macrophages from human peripheral blood
摘要
Abstract
Objective:To isolate monocytes from human peripheral blood mononuclear cells( PBMC) ,induce macrophages,and identify the function of macrophages.Methods:Monocytes were isolated from PBMC using magnetic activated cell sorting( MACS) anti-CD14 microbead.Sorted CD14+and CD14-cells were checked by flow cytometer to evaluate the efficiency of sorting.The sorted CD14+cells were cultured in IMDM media with 10%human AB serum and 10 ng/ml M-CSF for 7 days to generate macrophages,which were identified by morphological features and phagocytosis function.Results:A high purity of monocytes was obtained by MACS anti-CD14 microbead.The percentage of CD14+cells was 10% and 85.8% before and after sorting, respectively.The macrophages were approximately 40-45 μm in maximum diameter and had the fried egg colony morphological features after 7 days culture.The lymphoma ( Raji) cells were efficiently engulfed by macrophages.Conclusion: The high purity of CD14+monocytes is isolated from PBMC and monocyte-derived macrophages efficiently engulfed lymphoma cells.关键词
巨噬细胞/磁珠分选/培养/功能鉴定Key words
Macrophages/Magnetic activated cell sorting/Culture/Functional identification分类
医药卫生引用本文复制引用
林炜明,戴爱玲,尹会方,杨小燕..人外周血巨噬细胞培养及功能鉴定[J].中国免疫学杂志,2015,(1):86-89,4.基金项目
本文受福建省教育厅 JK 类项目（ No．JK2011053）、福建省科技计划重点项目（2013N0026）资助。 （）
