中国免疫学杂志Issue(2)：163-168,6.DOI:10.3969/j.issn.1000-484X.2015.02.005
丙烯酰胺对小鼠成体神经发生及GSK3β表达的影响
Influence of acrylamide on adult neurogenesis and expression of GSK3βin mouse
李凯凯 1谢炅芳 1王久涛 1宋玲珍 1张伟 1陈树林 1赵善廷1
作者信息
- 1. 西北农林科技大学动物医学院，杨陵 712100
- 折叠
摘要
Abstract
Objective:To investigate the influence of acrylamide ( ACR) on adult neurogenesis and expression of GSK 3βin mouse.Methods:Method Adult male Kunming mice were used and divided into two groups:control group and experimental poisoning groups,that were exposured to acrylamide by intraperitoneal injection.Brdu labeling and immunohistochemistry were used to investigate the proliferation of adult neural stem cells in the subgranular zone ( SGZ).BrdU/NeuN/GFAP triple labeling to investigate the survival and differentiation of newly generated cells.Detecting GSK3βexpression and distribution in Neuro-2a cells,the expression of GSK3βwas examined by using Western blot.Results:Compared with control mice ,lower number of BrdU-positive cells and less differentiated into neurons in ACR mice.Less neural stem cells survived ,but more glia cells were generated in the subgranular zone of acrylamide mice.Moreover,higher phosphorylated GSK 3β( Ser9 ) were detected in Neuro-2a cells and mouse dentate gyrus in ACR mice respectively .Conclusion:These results suggested that acrylamide inhibits neural stem cells proliferation and influences the survival and differentiation of newly generated cells.Acrylamide inhibits neurogenesis maybe through GSK 3βsignaling pathway.关键词
丙烯酰胺/成体神经发生/免疫组织化学/GSK3βKey words
Acrylamide/Adult neurogenesis/Immunohistochemistry/GSK3β分类
生物科学引用本文复制引用
李凯凯,谢炅芳,王久涛,宋玲珍,张伟,陈树林,赵善廷..丙烯酰胺对小鼠成体神经发生及GSK3β表达的影响[J].中国免疫学杂志,2015,(2):163-168,6.
