中国免疫学杂志Issue(2)：215-220,6.DOI:10.3969/j.issn.1000-484X.2015.02.016
鼠伤寒沙门菌SL1344株侵袭性蛋白B缺失株的构建及生物学特性
Construction and characterization of invasion protein B gene deleted mutant of Salmonella typhimurium SL1344 strain
摘要
Abstract
Objective:In order to develop a safer vaccine strain exploit Salmonella typhimurium vaccine strain .A ΔsipB mutant of Salmonella typhimurium SL 1344 strain was constructed.Methods: Firstly, the recombinant suicide plasmid containing the missing 585 bp sipB ( PREΔsipB ) was built by homologous recombination , and screenned by two-step method.Results: PCR and sequencing results showed that SL 1344ΔsipB was successfully constructed.It was no significant changes compared with SL 1344.But compared with the parent strains SL 1344 , the mutant strain had obvious change in its virulence , oral challenge of bacteria in mice revealed that LD50 of the mutant strain was 1.70 ×108 CFU,the toxicity reduced about 1.4%.The protection rate induced by the sipB mutant was 50%,and the serum antibody peaked 14 d post-immunization.Conclusion:The SL1344ΔsipB mutant was constructed suc-cessfully,and genetic stability ,significantly reduced virulence.The study provides a new approach for further study of the relationship between the gene and pathogenicity of Salmonella typhimurium.It is likely that the ΔsipB mutant could be adapted to develope attenuated Salmonella vaccine.关键词
鼠伤寒沙门菌SL1344/sipB基因/缺失株/生物学特性Key words
Salmonella typhimurium SL1344/sipB gene/Deleted mutant/Characteristics分类
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陈松彪,陈桂华,赵战勤,李静,郁川,何雷,张春杰,程相朝,李银聚,颜云飞,金修哲..鼠伤寒沙门菌SL1344株侵袭性蛋白B缺失株的构建及生物学特性[J].中国免疫学杂志,2015,(2):215-220,6.基金项目
本文受河南省科技攻关（112102110019）、河南科技大学博士启动基金项目（09001580）和河南科技大学青年科学基金项目（13000820）资助。 （）
