中国免疫学杂志Issue(3)：334-338,5.DOI:10.3969/j.issn.1000-484X.2015.03.010
益气养阴方对大鼠肺纤维化的干预作用及对 Smad2、Smad7蛋白的影响
Effect of Yiqiyangyin Decoction on pulmonary fibrosis rat and expression of Smad2/Smad7 protein
摘要
Abstract
Objective:To observe the effect of Yiqiyangyin Decoction( YQYY) on bleomycin-induced rats pulmonary fibrosis and the expression of Smad2 and Smad7.Methods:Sixty male SD rats were randomly divided into normal group,model group,YQYY group,hormone group,YQYY plus hormone group.The rats in the normal group injected saline via the trachea,the rest rats injected ble-omycin(5 mg/kg).From the second day of injection,YQYY group were treated with YQYY decoction(1 ml/100 g);hormone group were given 35%Prednisone acetate solution(1 ml/100 g);YQYY plus hormone group were treated with the mixture of YQYY decoction and hormone (1 ml/100 g);normal group and model group were given equivalent saline.After twenty-eight days,all the rats were killed,the rat lung histopathology was examined with HE staining and detect Smad2/Smad7 protein with immunohistochemical staining.Results:Compared with normal group, the model group rats alveolitis and pulmonary fibrosis scores were significantly increased;compared with model group,the hormone group,YQYY group,YQYY plus hormone group rats alveolitis and lung fibrosis scores were reduced,and the lung injury of YQYY group reduced obviously.The expression of Smad2 protein of model group in alveolar epithelial cells and the fibroblasts was significantly higher than normal group;compared with model group,the positive expression of Smad2 in hormone group,YQYY group,YQYY plus hormone group were reduced,the Smad2 of YQYY group was the lowest.Compared with normal group,the smad7 protein of model group decreased significantly;compared with model group,Smad7 protein expression in hormone group, YQYY group, YQYY plus hormone group were increased, the Smad7 expression of YQYY group was highest.Conclusion:Yiqiyangyin decoction has a good intervention and therapeutic effect on bleomycin-induced rats pulmonary fibrosis,its mechanism may be related to inhibiting the inflammatory reaction,reduce the formation and deposition of collagen and the intervention of Smad signal pathway.关键词
肺纤维化/益气养阴方/Smad2/Smad7Key words
Pulmonary fibrosis/Yiqiyangyin Decoction/Smad2/Smad7分类
医药卫生引用本文复制引用
冯佳,向阳,夏燕,蔡杰,陈安平,杨年安,龚书识,刘冬梅,袁林..益气养阴方对大鼠肺纤维化的干预作用及对 Smad2、Smad7蛋白的影响[J].中国免疫学杂志,2015,(3):334-338,5.基金项目
本文受风湿性疾病发生与干预湖北省重点实验室开放基金项目（OIR13013A）、湖北民族学院民大医院院内项目（MDYY201412）和湖北省医学领军人才培养工程专项经费资助。 （）
