中国免疫学杂志Issue(4)：480-484,489,6.DOI:10.3969/j.issn.1000-484X.2015.04.010
MyD88依赖途径介导云芝糖肽对荷瘤小鼠免疫调节的机制研究
Immunomodulatory effects of polysaccharopeptide on EAC tumor-bearing mice through MyD88-dependent signaling pathway
摘要
Abstract
Objective:To detect the effects of polysaccharopeptide(PSP) on MyD88-dependent signaling pathway in EAC tumor-bearing mice,and explore the immunomodulatory mechanism of PSP.Methods: Ehrlich′s ascites carcinoma(EAC) C57BL/6 mice were used to establish the animal model for solid tumor.Mice were divided into two groups:WT group and MyD88-/-group.After 22 days of treatment,quantitative real-time PCR( Q-PCR) ,Western blot and were used to detect the related gene and protein expression of TLR4 pathway in spleens,ELISA were used to detect the terminal effect factors secretion eyeball blood from each group.Results:Related genes and proteins of TLR4 pathway in spleen were up-regulated significantly from two groups.Compared with WT group, related genes and proteins in MyD88-dependent pathway(MyD88,TRAF6,NF-κB,AP-1)was down-regulated in MyD88-/-group(P<0.05).Meanwhile,There was no significant change of the related genes and proteins of MyD88-independent pathway(TRAM,TRIF)in MyD88-/-group( P>0.05 ) .The terminal effect factors secretion of IL-2, IL-6, IL-10, TNF-αand IFN-γin MyD88-/-group were decreased significantly compared with WT group(P<0.05).Conclusion:The immunoregulatory effect of PSP on EAC tumor-bearing mice may be implement through the regulation of MyD88-dependent signaling pathway.关键词
云芝糖肽/免疫调节/MyD88依赖途径/艾氏腹水癌Key words
Polysaccharopeptide/Immunoregulation/MyD88-dependent/EAC分类
医药卫生引用本文复制引用
冯子芳,汪志学,周丽菁,鲍依稀..MyD88依赖途径介导云芝糖肽对荷瘤小鼠免疫调节的机制研究[J].中国免疫学杂志,2015,(4):480-484,489,6.基金项目
本文受国家自然科学基金（81274144，81473388）资助。 （）
