中国免疫学杂志Issue(4)：497-501,508,6.DOI:10.3969/j.issn.1000-484X.2015.04.013
肿瘤相关成纤维细胞对舌鳞癌细胞功能的影响及机制研究
Effects of cancer associated fibroblasts on biological function of tongue cancer cell
摘要
Abstract
Objective:To elucidate the effects of CAFs on the proliferation,migration and invasion of oral cancer cells and those on tumor growth.Potential mechanism was discussed.Methods:CAFs and SCC-9 or CAL-27 were cocultured.The proliferation of oral cancer cells were detected by measuring the level of RFP.A interaction model between CAFs and SCC-9 or CAL-27 were established with Transwell chambers to abserve the effects of CAFs on the migration and invasion of OCCs.The conditioned medium collected from mono-cultured CAFs or SSC-9 cell,and co-culture were subjected to a cytokine antibody array.Volumes of xenografts were obtained and presented.Results:At day 5,significant difference was found in OCC proliferation between OCC-CAFs group and OCC group.The SCC-9 cell number was up to 4.41±0.38 times as many as the initial number in co-culture group and 3.21±0.35 times in SCC-9 group(P<0.05).The proliferation of CAL-27 was similar to that of SCC-9.SCC-9 cells migrated to the bottom in group SCC-9+CAFs were 2.6± 0.42 times as many as group SCC-9(P<0.05).CAL-27 cells migrated to the bottom in group CAL-27+CAFs were 3.11±0.46 times as many as group CAL-27 ( P<0.05 ) .The similar results were found in invasion analysis.OCCs invaded more quickly towards medium derived from co-cultured cells than that from OCCs alone.Three human cytokines(CCL2,CCL5 and IL-8)were significantly upregulated in conditioned medium from co-cultured cells compared with those from CAFs or SSC-9 alone.Six weeks after injection,we observed that SCC-9 mixed with CAFs generated tumors of greater volume than those generated from SCC-9 cells alone.Conclusion:CAFs Promote the Proliferation,Migration,Invasion of oral cancer cells and tumor growth.Cytokines(CCL2,CCL5 and IL-8)may responsible for the bi-ological function change of cancer cells.关键词
肿瘤相关成纤维细胞/口腔癌/侵袭/肿瘤生长Key words
Cancer associated fibroblasts/Oral carcinoma/Invasion/Tumor group分类
医药卫生引用本文复制引用
李霞,李晋芸..肿瘤相关成纤维细胞对舌鳞癌细胞功能的影响及机制研究[J].中国免疫学杂志,2015,(4):497-501,508,6.基金项目
本文受佛山科学技术学院博士启动项目资助 （）
