中国免疫学杂志Issue(5)：607-612,6.DOI:10.3969/j.issn.1000-484X.2015.05.007
细粒棘球蚴感染中阻断TGF-β1受体对淋巴细胞的影响
Effect of inhibition of TGF-β1 receptors on lymphocytes during infection of Echi-nococcus granulosus
摘要
Abstract
Objective:To investigate the effects of TGF-β1 on T lymphocytes of BALB/c mice infected with Echinococcus granulosus( E.granulosus ) in vitro.Methods: The inhibitor group:the spleen cells of BALB/c mouse were co-cultured with E.granulosus and SB525334.The control group:the spleen cells of BALB/c mouse were co-cultured with E.granulosus and PBS.The blank group:the spleen cells of BALB/c mouse were co-cultured with RPMI-1640 medium and SB525334.The lymphocytes were collected at 48 h post-infection.The T lymphocyte subsets, the number of CD4+CD25+T cells, the number of NK cells, and the expression of NKG2D receptor were detected by flow cytometry.The NK cell activity was determined with the lactate dehydrogenase leakage assay(LDH).Results:The inhibition the TGF-β1 receptors resulted in the increase of in the number of CD4+T cells,the decrease in the number of CD8+T cells,the increase of in the ratio CD4+/CD8+T cells,the decrease of in the number of CD4+CD25+T cells,the increase in the expression of the NKG2D receptors,the increase in the lysis rate of Yac-1 cells by NK cells,and a positive cor-relation between the expression of activity receptor NKG2D and killing activity of NK, which were mediated by E.granulosus.Conclusion: The inhibition of TGF-β1 receptors can enhance the immune response of T lymphocytes against E.granulosus infection in vitro.关键词
细粒棘球蚴/T细胞亚群/Treg细胞/NKG2DKey words
Echinococcus granulosus/T-lymphocyte subsets/Treg cells/NKG2D分类
医药卫生引用本文复制引用
印双红,杨文才,张俊波,陈小林,徐芳洁,侯隽,吴向未,陈雪玲,姚元勇,邢明明..细粒棘球蚴感染中阻断TGF-β1受体对淋巴细胞的影响[J].中国免疫学杂志,2015,(5):607-612,6.基金项目
本文受国家自然科学基金（81260412，81360453）、博士基金（2012BB018，trxyDH1504）及教合KY字［2011］005号资助。 （）
