中国免疫学杂志Issue(6)：764-768,5.DOI:10.3969/j.issn.1000-484X.2015.06.010
RNA干扰Fascin1表达抑制胶质瘤细胞U87 MG的侵袭、转移能力
Reversal effect of RNA interference targeting Fascin1 on migration and invasion of glioma cell line U87 MG
李培栋 1王新军 1单峤 1吴跃辉 1王振1
作者信息
- 1. 郑州大学第五附属医院神经外科，郑州 450052
- 折叠
摘要
Abstract
Objective:To detect the functional role of Fascin1 and its related molecular mechanisms in migration and invasion capacity of glioma cells,we utilized gene specific small interference RNA of Fascin1 in cell line U87 MG. Methods:Fascin1-siRNA or negative siRNA was transfected into U87 MG cells of control group or experiment group. Transwell method was employed to assess the migration and invasion capacity of glioma cells. Western blot analysis was used to detect the protein expression of Fascin1,pAKT and pSTAT3. The impact of PI3K/AKT pathway and STAT3 pathway on migration and invasion of U87 MG cells was verified,via applying LY294002 and LY294002,which was inhibitor of the two pathways respectively. Results:As compared to control groups,the migration and invasion capacity of transfected glioma cells were attenuated about 52% or 43%(P<0. 05),accompanied with the decreased phos-phorylation of AKT and STAT3. As utilizing the inhibitors of AKT and STAT3,attenuated migration and invasion capacity of U87 MG cells were observed. Conclusion:Down-regulated expression of Fascin1 could suppress the migration and invasion capacity of U87 MG cells by inhibiting the phosphorylation of PI3K/AKT pathway and STAT3 pathway.关键词
RNA干扰/Fascin1/胶质瘤/U87 MG/侵袭转移Key words
RNA interference/Fascin1/Glioma/U87 MG cell line/Migration and invasion分类
医药卫生引用本文复制引用
李培栋,王新军,单峤,吴跃辉,王振..RNA干扰Fascin1表达抑制胶质瘤细胞U87 MG的侵袭、转移能力[J].中国免疫学杂志,2015,(6):764-768,5.
