中国免疫学杂志Issue(6)：798-802,5.DOI:10.3969/j.issn.1000-484X.2015.06.017
Ⅰ型单纯疱疹病毒单克隆抗体的制备及其应用
Preparation and application of monoclonal antibodies against Herpes simplex virus-1
尹炳谦 1贾继宗 1赵风强 2韩金乐 1黄承浩 2叶祥忠 1张景海2
作者信息
- 1. 沈阳药科大学生命科学与生物制药学院，沈阳 110016
- 2. 北京万泰生物药业股份有限公司，北京 102206
- 折叠
摘要
Abstract
Objective:To prepare and screen monoclonal antibodies against Herpes simplex virus-1(HSV-1),and develop a double antibody sandwich quantitative enzyme-linked immunosorbent assay( Q-ELISA) for detection of HSV-1 particle. This method was used to control the quality of viral particle in the developing and manufacturing process of HSV-1. Methods: BALB/c mice was immunized with HSV-1 to prepare monoclonal antibodies. A double antibody sandwich Q-ELISA was developed to determine concentration of HSV-1 particle,which was based on the neutralizing monoclonal antibody 1F6 as capture antibody,and 2B1 as HRP-conjugated antibody. The performance of the reagent was evaluated,including specificity,sensitivity,precision,accuracy and linear. And the relation between the amount of virus detected by this method and the virus titer was analyzed by regression analysis method. Results: The Q-ELISA for HSV-1 particle was developed. The quantitation scope was 0. 125-2 μg/ml, the coefficient correlation was 0. 995 5, the limit of detection was 0. 125 μg/ml, the recovery was between 85. 6% and 107. 1%, the variation coefficient was lower than 10%, and the reagent does not react with other samples except HSV-1 antigen. This method has a good correlation with virus titer. Conclusion:The Q-ELISA for HSV-1 particle was successfully developed,which provide a new approach for rapid and quantitative detection of HSV-1 antigen.关键词
HSV-1病毒/溶瘤/颗粒完整性/病毒滴度Key words
HSV-1/Oncolytic/Particle integrity/Virus titer分类
医药卫生引用本文复制引用
尹炳谦,贾继宗,赵风强,韩金乐,黄承浩,叶祥忠,张景海..Ⅰ型单纯疱疹病毒单克隆抗体的制备及其应用[J].中国免疫学杂志,2015,(6):798-802,5.
