中国免疫学杂志Issue(7)：890-894,5.DOI:10.3969/j.issn.1000-484X.2015.07.005
JMJD3参与IFN-α和TLR7诱导的B细胞活化及凋亡
JMJD3 participates in activation and apoptosis of IFN-αand TLR7-induced B cells
摘要
Abstract
Objective:To explore the effect of histone demethylase JMJD3 on B cell activation and apoptosis.Methods:B cells were sorted and purified from the peripheral blood of healthy people and SLE patients by using magnetic bead.After B cells were treated with IFN-αor R848 or IFN-α+R848,the percentages of CD86+B cells,CD69+B cells,CD86+Annexin V+B cells and CD69+Annexin V+B cells were detected by flow cytometry.The expression of JMJD3 was detected by Real Time PCR and Western blot.Results:The purity of sorted B cells was up to 95%.IFN-αenhanced both the activation and apoptosis and the JMJD3 expression of TLR7-activated B cells.The expression of JMJD3 was dependent on MAPK signal pathway,but not the NF-κB signaling pathway.Moreover,JMJD3 was highly expressed in B cells of peripheral blood from SLE patients compared to those from healthy people.Furthermore,JMJD3 inhibitors could inhibit the activation and apoptosis of IFN-αand R848 activated B cells.Conclusion:JMJD3 participated in the activation and apoptosis of IFN-αand TLR7-induced B cells, suggesting JMJD3 inhibitors may possess therapeutic effect for alleviating symptom of SLE.关键词
B细胞/干扰素-α/Toll样受体7/组蛋白去甲基化酶JMJD3Key words
Bcells/IFN-α/Toll-like receptor 7/Jumonji domain containing 3 histon lysine demethylase分类
医药卫生引用本文复制引用
樊竑冶,李宝辉,刘飞,任德善,侯亚义,周长林..JMJD3参与IFN-α和TLR7诱导的B细胞活化及凋亡[J].中国免疫学杂志,2015,(7):890-894,5.基金项目
本文为第53批中国博士后科学基金面上资助项目(2013M531331）。 （）
