中国麻业科学Issue(2)：57-63,7.
苎麻 Genomic －SSR 与 EST －SSR 分子标记遗传差异性分析
Genetic Differences in Ramie Analyzed between Genomic -SSR and EST -SSR
摘要
Abstract
In order to clarify the genetic differences among different SSRs,Genomic -SSR and EST-SSR were used to analyze the genetic diversity of 19 important ramie genetic resources.According to the statistical results,the average observed -alleles detected by Genomic -SSR was 3.4643,effective alleles was 2.1982,shannon was 0.8864,heterozygosity was 0.5254,expected heterozygosity was 0.5172.The average observed -alleles detected by EST -SSR was 2.3333,effective alleles was 1.9438,shannon was 0.7120,heterozygosity was 0.5128,expected heterozygosity was 0.4778.There were no significant differences in effective number of alleles (Ne)and observed heterozygosity (Ho)be-tween Genomic -SSR and EST -SSR.Genomic -SSR Shannon index was significantly higher than that of EST -SSR.The above results showed there were significant genetic differences in marker polymor-phism and genotype identification between Genomic -SSR and EST -SSR,but the two kinds of markers could also effectively identify different genotypes.Genomic -SSR had priority for molecular identifica-tion,construction of high -density genetic linkage map and analysis of genetic diversity,EST -SSR had more advantages of studying the genetic diversity among close ramie species,evolutionary analysis,ge-netic map and comparative genomics.关键词
苎麻/遗传差异性/分子标记/Genomic -SSR/EST -SSRKey words
ramie/genetic differences/molecular marker/Genomic -SSR/EST -SSR分类
农业科技引用本文复制引用
刘晨晨,栾明宝,陈建华,王晓飞,许英,孙志民..苎麻 Genomic －SSR 与 EST －SSR 分子标记遗传差异性分析[J].中国麻业科学,2015,(2):57-63,7.基金项目
国家自然科学基金（31471547；30900913）；湖南省自然科学基金（13JJ4116）；国家科技支撑计划 （）
