中国麻业科学Issue(2)：87-94,8.
同源重组整合 vgb 基因提升枯草芽孢杆菌亚麻脱胶能力
Improvement of Flax Degumming by Chromosomal Integration of Vitreoscilla Hemoglobin Gene (vgb) in Bacillus subtilis
摘要
Abstract
The gene Vitroscilla Hemoglobin was integrated and expressed in Bacillus subtilis to en-hance the effect of degumming by improving pectinase production.Promoter p43 and gene amyE,pSK -vgb (+)were integrated into expression vector.Through the method of homologous recombination, gene vgb was integrated into Bacillus subtilis A5,and then got a vgb integrated expression strain named Bacillus subtilis A5 -vgb (+).The result was identified by PCR analysis and CO -spectral analysis.A5 -vgb (+)strain was applied in flax degumming,shake flask experiment was used to study the func-tion of protein VHb in production of Pectinase.X -ray diffraction (XRD)and canning electron micro-scope (SEM)were uesd to analysis the effect of degumming.PCR analysis and CO -spectral analysis showed that the gene vgb was succeed in being integrated into Bacillus subtilis A5 and protein VHb had bioactivity.The results of shake flask experiment showed that the expression of gene vgb could improve Pectinase production,and the activity of Pectinase was 29.5% higher than that of the control under 150 rpm,37℃,pH =8.0,16 hours .The data of XRD and SEM showed that being compared with the strain without gene vgb,the degumming effect of A5 -vgb (+)strain improved 0.55% in crystallinity, and had a smoother surface.To some extent,the VHb could enhance the degumming effect of Bacillus subtilis A5.关键词
生物脱胶/果胶酶/枯草芽孢杆菌/vgb 基因/同源重组Key words
bio -degumming/Pectinase/Bacillus subtilis/gene vgb/homologous recombination分类
生物科学引用本文复制引用
张懿翔,丁若篧,陈婷,郁崇文,张兴群..同源重组整合 vgb 基因提升枯草芽孢杆菌亚麻脱胶能力[J].中国麻业科学,2015,(2):87-94,8.基金项目
中国博士后科学基金面上资助（2014M561386）；国家麻类产业技术体系建设专项资金资助项目（CARS －19）；中央高校基本科研业务专项资金（14D110524）；大学生“小平科技创新团队” （）
