农学学报Issue(12)：95-98,4.
3种桑蟥基因组DNA提取方法比较研究
Comparison of Three Methods for Genomic DNA Extraction from the Rondotiamenciana Moore
摘要
Abstract
To explore an efficient, stable, low-cost DNA extraction method from Rondotia menciana Moore, genomic DNA was extracted using CTAB, protein enzyme K and salting-out method, respectively. The extracted genomic DNA samples of three methods were tested using photometric analysis and COI gene amplification. The results showed that, all the three methods can be used for the total DNA extraction, which are suitable for the PCR amplification, but genome DNA extracted by CTAB method and protein enzyme K method were higher in purity and production, degraded slightly. So we should select the proper method according to the objective and condition in the study.关键词
桑蟥/DNA提取/CTAB法/蛋白酶K法/盐析法Key words
Rondotia menciana Moore/DNA Extraction/CTAB Method/Protein Enzyme K Method/Salting-out Method分类
农业科技引用本文复制引用
王钰婷,汪泰初,李瑞雪,王伟..3种桑蟥基因组DNA提取方法比较研究[J].农学学报,2014,(12):95-98,4.基金项目
安徽省农科院院长青年创新基金(13B0632)；国家蚕桑产业技术体系合肥综合试验站(CARS-22-SYZ09)；安徽省农科院创新团队项目(11C0610)。 （13B0632）
