农学学报Issue(5)：25-28,4.
高效液相色谱法同时检测虫草制品中腺苷和虫草素含量的研究
Determination of Adenosine and Cordycepin in Cordyceps Preparation by HPLC
摘要
Abstract
To establish the quantitative analysis method for adenosine and cordycepin in Cordyceps sinensis by HPLC. The sample was extracted with pure water under ultrasonic for 40 min. After centrifugation and filtration, the extract was analyzed by HPLC with C18 column at 1.0 mL/min and detected at 260 nm wavelength. The mobile phase was composed of pure water and methanol(85:15, V/V) and the column temperature was 30℃. This method showed the LOD was 0.2 μg/mL, LOQ was 1.0 μg/mL. The average recoveries of adenosine and cordycepin were 94%-103% and RSD bellow 3%. The method had high sensitivity, selectivity and simple operation, and can be used for testing the adenosine and cordycepin.关键词
虫草制品/高效液相色谱/腺苷/虫草素Key words
Cordyceps Preparation/HPLC/Adenosine/Cordycepin分类
化学化工引用本文复制引用
黄丽俊,李利东,宓晓黎..高效液相色谱法同时检测虫草制品中腺苷和虫草素含量的研究[J].农学学报,2015,(5):25-28,4.基金项目
无锡市社会发展科技支撑计划“构建食品中非法添加物快速检测关键技术集成及应用”(CSE01N1205)。 （CSE01N1205）
