中国脑血管病杂志Issue(8)：420-423,4.DOI:11.3969/j.issn.1672-5921.2014.08.007
猪胰弹力蛋白酶血管外消化法制作兔颈总动脉梭形动脉瘤
Inducing common carotid fusiform aneurysms in rabbit with the porcine pancreatic elastase extravascular digestion method
摘要
Abstract
Objective To investigate the feasibility and effectiveness of inducing rabbit common carotid fusiform aneurysms via the common carotid extravascular digestion method. Methods Sixteen New Zealand white rabbits were randomly assigned into either an experiment group ( n=12 ) or a control group (n=4). Porcine pancreatic elastase 80-400 U were used to incubate and digest 2 to 4 cm segment of artery distal to the origin of right common carotid artery. One week after modeling,intravenous angiography was performed and the length and width of fusiform dilatation of common carotid artery were measured. The fusiform dilated artery was examined with hematoxylin and eosin staining and the vascular morphological changes were observed with scanning electron microscope. Isotonic saline solution was used to incubate common carotid arteries of the 4 New Zealand white rabbits in the control group. After one week,the same method was used to observe the lumen of common carotid artery and intimal changes. Results After the digestion of common carotid artery adventitia,the angiography of 12 New Zealand white rabbits of the experimental group revealed fusiform dilatation of common carotid artery of the 10 model rabbits. The widest diameter of the fusiform artery was 3. 70 ± 0. 32 mm;two rabbits had common carotid artery occlusion. Compared with the control group,the right common carotid artery diameter enlarged significantly in the experimental group (1. 80 ± 0. 16 mm,P<0. 01). The HE staining showed that the lumen widened, adventitia and media reduced. Scanning electron microscope showed intimal inflammatory injury and thrombus attachment. Conclusion Using porcine pancreatic elastase to digest the adventitia of common carotid artery can make fusiform dilatation of common carotid artery in rabbits. Using this method may effectively induce a model of fusiform aneurysm,and it has certain feasibility.关键词
颈总动脉/动脉瘤/猪胰弹力蛋白酶/血管造影术,数字减影/兔/梭形动脉瘤Key words
Common carotid artery/Aneurysm/Porcine pancreatic elastase/Angiography, digital subtraction/Rabbits/Fusiform aneurysm引用本文复制引用
李子付,江国权,方兴根,张连富,方亦斌,黄清海,刘建民..猪胰弹力蛋白酶血管外消化法制作兔颈总动脉梭形动脉瘤[J].中国脑血管病杂志,2014,(8):420-423,4.基金项目
上海市科学技术委员会科研计划项目(13140903201) （13140903201）
上海市科委实验动物专项基金(12140901801) （12140901801）
上海市科委基础重点项目(11JC1415800) （11JC1415800）
