中国农业科技导报Issue(6)：44-51,8.DOI:10.13304/j.nykjdb.2014.339
赤芝菌株的SCAR标记鉴别
Identification of SCAR Molecular Marker Technology among Ganoderma lucidum Strains
摘要
Abstract
Ganoderma lucidum is an important medical fungi and has wide varieties. At present, the edible fungus management system is not yet perfect, so it is an urgent need to establish an effective way for rapid identification of Ganoderma lucidum strains to stable strains quality. Based on SRAP ( sequence⁃related amplified polymorphism ) analysis on cultivated strains of Ganoderma lucidum, specific SRAP marker bands belonged to several tested strains were converted into more stable SCAR ( sequence characterized amplified region) markers. Seventeen SCAR markers were obtained and 23 strains were classified into 4 groups using clustering analysis under genetic distance of 0. 63, and among them 19 strains were belonged to one group under the distance value of 0�50. Multiple markers comprehensive identification method for Ganoderma lucidum strains was established through combining usage of 9 SCAR markers, and the tested 23 strains were effectively identified. This showed that SCAR molecular markers could well explain the genetic relationship between Ganoderma lucidum strains, and it was a rapid, stable and accurate method to identify Ganoderma lucidum strains.关键词
赤芝/SRAP标记/SCAR标记Key words
Ganoderma lucidum/SRAP marker/SCAR marker分类
农业科技引用本文复制引用
陈凌华,洪自同,程祖锌,谢宝贵,郑金贵..赤芝菌株的SCAR标记鉴别[J].中国农业科技导报,2014,(6):44-51,8.基金项目
福建省中青年教师教育科研项目（ JB13287）资助。 （）
