中国农业科学Issue(8)：1652-1656,5.
密花胡颓子果实主要类胡萝卜素组分及含量分析
Determination of Carotenoids in the Fruit of Elaeagnus conferta Roxb. by High Performance Liquid Chromatography
摘要
Abstract
Objective]The berry of Elaeagnus umbellata Thunb. was found to accumulate lycopene up to 54 mg per 100 g of fresh weight, which is about 17 times higher than that of tomato. However, little is known about the carotenoid composition and content in the big and good tasty flesh of E. conferta Roxb., also a member of Elaeagnaceae family. This study aimed to analyze carotenoid components and contents in the fruit of E. conferta Roxb. by high performance liquid chromatograph(HPLC)method.[Method] Methanol:chloroform (1:2 v/v) was used to extract carotenoids from ripe fruits of E. conferta Roxb.. The separation was carried on a YMC C30 (4.6 mm×250 mm, 5 µm, column temperature 235℃) and an injection volume was 20μL. Methanol (A), 80%methanol containing 0.2%ammonium acetate (B) and methyl tert-butyl ether (C) were used as the mobile phase at a flow rate of 1.0 mL·min-1 through gradient elution: (1) 0-6 min, 95%A+5%B; (2) 7-11 min, 80%A+5%B+15%C; (3) 12-32 min, 30%A+5%B+65%C;(4) 33 min to end, 95%A+5%B. The eluent was detected at 244 nm. [Result] Lycopene andβ-carotene were two major carotenoids in the flesh of E. conferta Roxb., and their content differed markedly between two genetic genotypes. Theβ-carotene content in Detianmihuahutuizi 1 was up to (231.84±16.00) µg·g-1DW, which is one of the highest ones in the known species. The flesh of Detianmihuahutuizi 2 contained lycopene of (1 054.10±29.19) µg·g-1DW, which is about seven times higher than that of tomato.[Conclusion]The results showed that the flesh of E. conferta Roxb. accumulated abundant lycopene andβ-carotene, which endow E. conferta Roxb. as a wilding fruit resource with great development and application value. Meanwhile, E. conferta Roxb. also is an ideal research material for studying molecular mechanism of high accumulation of lycopene andβ-carotene in fruit.关键词
密花胡颓子/番茄红素/β-胡萝卜素/高效液相色谱Key words
Elaeagnus conferta Roxb/lycopene/β-carotene/high performance liquid chromatography引用本文复制引用
胡海涛,金晓琴,成龙平,杨莉,陈建华,番汝昌,杨玲..密花胡颓子果实主要类胡萝卜素组分及含量分析[J].中国农业科学,2014,(8):1652-1656,5.基金项目
国家自然科学基金项目（31071775）、浙江省重中之重学科“现代农业生物技术与作物病害防控”开放基金资助项目 （）
