中国农业科学Issue(9)：1836-1846,11.DOI:10.3864/j.issn.0578-1752.2014.09.019
贵州省烟青虫遗传多样性
Genetic Diversity ofHelicoverpa assulta (Lepidoptera：Noctuidae) in Guizhou Province
摘要
Abstract
Objective The objectives of the study are to explore genetic differentiation of oriental tobacco budworm (Helicoverpa assulta) among different geographic populations in Guizhou, uncover the differential law and mechanism, and to provide a scientific basis for the pest monitoring and integrated control.[Method] PCR amplifications were performed by 6 pairs of SSR primers that were screened out from 43 pairs of primers from closely-related species, using DNA from 30H. assulta geographic populations in Guizhou as template. The PCR products were subjected to 8% non-denaturing polyacrylamide gelelectrophoresis and visualized by silver staining. Genetic diversity was analyzed byPopGene Version 1.32, and the UPGMA dendrogram was constructed by using MEGA 5.0 based on the Nei’s genetic distance. The correlation between genetic and geographic distances as well as between genetic identity and elevation were analyzed by using Mantel.[Result] The observed and effective number of alleles ranged from 3 to 8 with an average of 5 and from 1.4498 to 2.2219 with an average of 1.8594, respectively. Shannon’s information index ranged from 0.5310 to 1.0609 with an average of 0.8423. The observed (Ho) and expected heterozygosity (He) varied from 0.0260 to 0.2672 with a mean of 0.1239 and from 0.3123 to 0.5520 with a mean of 0.4539, respectively. TheHe was higher than theHo, suggesting that all populations were mainly in homozygous condition.FISchanged from 0.0798 to 0.7906 with a mean of 0.2801;FIT changed from 0.4842 to 0.9731 with a mean of 0.7809. BothFISandFITwere positive, indicating that there existed inbreeding in the all populations of H. assulta from tobacco-growing areas of Guizhou.FST ranged from 0.3897 to 0.9256 and gene flow (Nm) was less than 1, which reflected high genetic differentiation and a low level ofNm among all the populations. Genetic distance was in a range of 0.0068-2.5193 and genetic identity was in a range of 0.1051-0.9933. The minimum genetic distance (0.0068) and the maximum genetic identity (0.9933) were observed between Songtao and Yinjiang populations, whereas the maximum genetic distance (2.5193) and the minimum genetic identity (0.1051) were observed between Daozhen and Hezhang populations. The UPGMA phylogenetic analysis showed that the 30 geographic populations in Guizhou were clustered into three groups. The clustering had no significant correlation with the geographic distribution and only in a few populations the genetic differentiation had relationship with geographic distance. Mantel tests demonstrated that there was no significant correlation between genetic and geographic distances as well as between genetic identity and elevation.[Conclusion] There exists a high level of genetic diversity among different geographic populations ofH. assulta in Guizhou. All the populations are highly differentiated and the genetic differentiation primarily occurred between populations. Geographic isolation has no significant effect on the genetic differentiation.关键词
贵州/烟青虫/微卫星标记/遗传多样性/遗传分化Key words
Guizhou/Helicoverpa assulta/microsatellite marker/genetic diversity/genetic differentiation引用本文复制引用
师沛琼,杨茂发,吕召云,李尚伟,廖启荣,商胜华,徐进,吴怡蓓..贵州省烟青虫遗传多样性[J].中国农业科学,2014,(9):1836-1846,11.基金项目
中国烟草总公司贵州省公司2010年度科学研究技术开发项目计划 （）
