中国农业科学Issue(10)：2062-2068,7.DOI:10.3864/j.issn.0578-1752.2014.10.020
一个甘蓝型油菜胚珠败育突变体的形态鉴定及生理特性分析
Morphologic and Physiologic Characterization of an Ovule Abortion Mutant in Brassica napus
摘要
Abstract
[Objective]The development of ovule is closely related with seed-setting and seed weight, which are components of seed yield in rapeseed. The investigation of morphologic and physiologic characterization of abortion mutant will benefit understanding the mechanism of development of ovule. [Method] In this study, OVA047,an ovule abortion mutant, was characterized in histological anatomy and investigated for the effects of nutrition of boron and temperature in Chongqing. Acetic red dyeing method was used to identify pollen fertility of OVA047. The buds of OVA047 which were about to open were selected for artificial emasculation and pollination. The ovary samples were taken and the pollen tube elongation was observed every 4 hours after artificial pollination. Foliar boron treatments were applied for one month seedlings of the normal materials and OVA047. Vegetative growth, pollen fertility and seed setting rate were investigated. Buds were artificially stripped, emasculated and pollinated, pollination time was tagged, statistical analysis was made for abortive rate in different periods after pollination. Daily average temperature and daily maximum temperature were recorded and the correlation between temperature and abortive rate was investigated. The data obtained were analyzed by using software SAS.[Result] Normal sexual organisms and pollination could be detected and the pollen dye ability rate was 95.90%.Pollen tube could germinate and reach the ovary. The development of ovule was found in good by anatomy on 7 day and 14th day after pollination, but most of ovules stopped growth 20 days after pollination in OVA047, especially at the top of inflorescence with 0.8 seeds per pod 35 days after pollination, 4%of the control. The content of boron in leaf was increased with the content of foliar-applied boron treatment in both normal material and OVA047. No significant differences were detected between OVA047 and control in the content of boron in leaf in the same treatment of boron fertilization. However, in different treatments of boron fertilization, all the OVA047 still exhibited low seed-setting (3.2) and around 60%of ovule abortion ratio in the inflorescence while the control still exhibited normal seed-setting (29). Two years observation results showed that large-scale ovule abortion phenomenon at the top, middle and basal of the inflorescence occurred in the significant temperature rise period when OVA047 grown in Chongqing, the later ovule abortion was increased with the daily temperature. A strong and positive association was found between ovule abortive rate of OVA047 and daily maximum temperature.[Conclusion]The ovule abortion of OVA047 is not due to fertilization failure and possible does not relate with boron fertilization, but associate with temperature.关键词
甘蓝型油菜(Brassica napus)/胚珠败育/硼/温度Key words
Brassica napus/ovule abortion/boron/temperature引用本文复制引用
许汪洁,傅鹰,董红利,陈致富,张沁芸,毛少帅,贺亚军,钱伟..一个甘蓝型油菜胚珠败育突变体的形态鉴定及生理特性分析[J].中国农业科学,2014,(10):2062-2068,7.基金项目
国家自然科学基金项目（31301351）、西南大学博士基金项目 （）
