中国农业科学Issue(16)：3169-3175,7.
灰葡萄孢犬尿氨酸单氧酶基因BcKMO调控病菌致病力的机制分析
Mechanism Analysis of Kynurenine 3-monooxygenase GeneBcKMO in Regulation of Pathogenicity inBotrytis cinerea
摘要
Abstract
[Objective]The objective of this study is to reveal the molecular mechanism of kynurenine 3-monooxygenase gene BcKMO in regulating pathogenicity ofBotrytis cinerea and lay a foundation for clarifing the pathogenic mechanism ofB. cinereain the future.[Method]The activities of polymethylgalacturonase (PMG), endopolygalacturonase (PG), cellulase (CX), polygalacturonic acid transeliminase (PGTE) and pectin methyl transelimination enzyme (PMTE) were analyzed in the wild-type strain (WT), the BcKMO gene ATMT mutant (BCG183) and gene complement mutant (BCG183/BcKMO) by DNS and Hoffman methods. The toxin was isolated from WT, BCG183 and BCG183/BcKMO and the activity of toxin was analyzed. Acid production assays was performed on PDA medium with bromothymol blue. Penetrability of WT, BCG183 and BCG183/BcKMO was detected with onion epidermis spread on PDA medium. Real-time PCR was used to measure the transcription levels of pathogenicity-related genes, e.g.,Bac, Bcg2,Bcg3, PkaR,Bmp1,Sak1,Bcreg1,Bos1,Bcp1,Ras2,Bcpg1, andSod1 in WT, BCG183 and BCG183/BcKMO.[Result]The activity of PMG and PG in mutant BCG183 were significantly higher than that of WT and BCG183/BcKMO. The CX, PGTE and PMTE activities were no significant difference while compared with WT and BCG183/BcKMO. The toxin activity of mutant BCG183 was significantly higher than that of WT and BCG183/BcKMO. The acid production of the mutant BCG183 significantly decreased. The penetrability of mutant BCG183 appeared no significant difference compared to WT and BCG183/BcKMO. The expression level of pathogenicity-related genes, i.e.,Bac,Bcg2,Bcg3, PkaR,Bmp1,Sak1,Bcreg1,Bos1,Bcp1,Ras2,Bcpg1, and Sod1, was obviously up-regulated in mutant BCG183.[Conclusion]TheBcKMO gene is involved in regulating cell wall degradation enzyme activity, toxin activity, acid production, penetrability, and the expression of pathogenicity- related genes inB. cinerea.关键词
灰葡萄孢/BcKMO/突变体/致病力Key words
Botrytis cinerea/BcKMO/mutants/pathogenicity引用本文复制引用
李培芬,赵福鑫,董丽萍,郑会欣,赵斌,韩建民,邢继红,董金皋..灰葡萄孢犬尿氨酸单氧酶基因BcKMO调控病菌致病力的机制分析[J].中国农业科学,2014,(16):3169-3175,7.基金项目
河北省科技支撑计划 （）
