中国农业科学Issue(17)：3483-3491,9.
沙门氏菌和聚肌胞处理条件下鸡免疫相关候选基因mRNA表达特性
Transcription Profiles of Immune-Related Genes in Chickens Infected bySalmonella Enteritidis and Poly(I:C)
摘要
Abstract
Objective] Genome-wide association studies were performed by using the chicken 60k high density SNP array for nine immune traits in Beijing-You chicken and identification of candidate genes and loci responsible for these traitswere identified. Here, by treating Beijing-You chicken with polyinosinic acid-polycytidylic acid, Poly(I:C) and Salmonella enteritidis (SE), several candidate genes were further studied based on authors’ GWAS result, includingCD1b,BMA1(B locus M alpha chain 1),TRIM27 (tripartite motif-containing 27) andZNF692(zinc finger protein 692).[Method] Eighty 12-d-old Beijing-You Chickens were divided into three groups: the control group, Poly(I:C) treatment and SE treatment groups. All the experimental birds were reared in isolated facility. The treatment groups were, respectively, injected intramuscularly into the breast with 0.5 mL of Poly(I:C) and SE bacterial suspension containing 108 CFU and the control group was given 0.5 mL saline. Chickens were sacrificed at 12 h, 24 h, 3 and 6 days of post infection (DPI). Blood samples were collected and serum was stored. The bursa of Fabricius, thymus and spleen were rapidly removed to test inflammatory factors and gene expression.[Result] The weight was significantly lower than control group 24 h post infection (P<0.01) and temperature of chickens was significantly changed in 24 h after treatments. In serum, concentrations of IFN-α, IL-4 and IL-6 were significantly higher than the control and reached a peak at 24 h or 3 d (P<0.01). TNF-αincreased in all periods and significantly higher than the control group after 3 days. The expression of candidate geneCD1b was not tissue-specific, butBMA1,TRIM27 andZNF692 were highly expressed in thymus and bursa fabricius. In thymus, the expression of CD1bwas significantly different between two treatments at 12 h and 24 h post infection. When treated with SE, the expression of CD1b was significantly increased and reached a peak at 24 h (P<0.01). The mRNA expression ofBMA1 was significantly different between two treatment groups in 12 h and 3 d post infection, when treated with Poly(I:C), the expression at 12 h post infection was lower than the SE group, but it was significantly higher than SE group (P<0.01). The expression ofTRIM27 on 6 DPI in Poly(I:C) treatment was significantly higher than control group, expression ofZNF692 was not different among three groups. In bursa of Fabricius, the expression ofCD1b was the highest at 12 h after infection. There were no differences in the expression of genesBMA1 andTRIM27 between two treatments. The expression ofZNF692 was higher on 1 DPI in SE treatment than Poly(I:C), and also the highest on 3 DPI in both groups.[Conclusion]CD1b,BMA1,TRIM27 andZNF692 might be involved in the immune response after treating withS. enteritidis and Poly(I:C).CD1b gene had an important role in the early stage ofSE infections.BMA1 andZNF692 were involved in immune response of thymus and bursa.关键词
CD1b/BMA1/TRIM27/ZNF692/mRNA表达/鸡Key words
CD1b/BMA1/TRIM27/ZNF692/mRNA expression/chickens引用本文复制引用
孙艳,李建超,李鹏,郑麦青,刘冉冉,李庆贺,文杰,赵桂苹..沙门氏菌和聚肌胞处理条件下鸡免疫相关候选基因mRNA表达特性[J].中国农业科学,2014,(17):3483-3491,9.基金项目
国家科技支撑计划项目（2011BAD28B03）、国家家养动物种质资源平台项目（2014）、国家肉鸡产业技术体系项目（CARS-42）、基本科研业务费 （）
