中国农业科学Issue(22)：4488-4494,7.DOI:10.3864/j.issn.0578-1752.2014.22.014
基于SSR标记的中国亚洲韧皮杆菌种群结构研究
Population Structure of‘Candidatus Liberibacter asiaticus’ in China Revealed by SSR Markers
摘要
Abstract
[Objective] ‘Candidatus Liberibacter asiaticus’ is the pathogeny of Citrus Huanglongbing. The purpose of this research was to investigate the population structure of ‘Candidatus Liberibacter asiaticus’ in China by multi SSR (simple sequence repeat) loci. [Method] To identify the polymorphism of SSR loci in the genome of Ca. L. asiaticus, 25 reported SSR primer sets were tested using DNA samples obtained from HLB-affected plants from 8 provinces of China. The selected SSR loci were used to analyze 285 samples by PCR and PAGE electrophoresis. The software Quantity One 4.5.0 was applied to estimate the base pairs of the amplicons. The polymorphism of 285 samples in the selected loci were evaluated via the software PopGen <br> version 1.31, and the clustering analysis was performed by software Powermarker 3.25 and structure 2.3.4.[Result]A total of 5 SSR loci were identified, with Nei’s gene diversity index ranging from 0.1542 to 0.9556. The locus LasA showed high diversity. Its effective number of allele (NE) and Nei’s gene diversity (H) were 22.5 and 0.9556, respectively. Analysis on population structure of Ca. L. asiaticus of China from different geographical samples revealed that the isolates from Yunnan province had high diversity, (NE=5.7, H=0.6580). The genetic distance of different geographical populations ranged from 0.0236 to 0.5786 and genetic identity ranged from 0.5607 to 0.9767. The longest genetic distance and lowest genetic identity were identified from the geographical population between Guangxi and Sichuan. The clustering analysis indicated all isolates from China were clustered into two groups. One is from Sichuan and Yunnan, the other is from Fujian, Zhejiang, Guizhou, Guangxi, Guangdong and Jiangxi. Structure analysis also showed the populations of Yunnan and Sichuan were constituted nearly by a single group and the rest of them were made up by mixed groups. [Conclusion]There are possible two kinds of different population structures of Ca. L. asiaticus in China by using the selected SSR markers and the results of this study will provide better insight on the origin and epidemiology of HLB in China.关键词
柑橘黄龙病/SSR/种群结构Key words
Citrus Huanglongbing/SSR/population structure引用本文复制引用
黄爱军,苏华楠,王雪峰,唐科志,李中安,周常勇..基于SSR标记的中国亚洲韧皮杆菌种群结构研究[J].中国农业科学,2014,(22):4488-4494,7.基金项目
中央高校基本科研业务创新团队项目（XDJK2014A001）、黄龙病溃疡病行业公益项目 （）
