中国农业科学Issue(22)：4540-4551,12.DOI:10.3864/j.issn.0578-1752.2014.22.020
不同产地野生黑果枸杞资源果实多酚组成分析
The Polyphenols Composition of Lycium ruthenicum Murr. from Different Places
摘要
Abstract
[Objective] Lycium ruthenicum Murr. is grown in the northwest of China, and its fruits is used in medicinal foods <br> for treatments of heart disease, abnormal menstruation and menopause. In the past few years, the polyphenols (especially anthocyanins) are proved to be the main effective components in Lycium ruthenicum Murr. Composition determination and evaluation of anthocyanin and other polyphenols of wild L. ruthenicum fruit in different origin, so as to provide the basis for the breeding and utilization of it.[Method]The contents of total polyphenols, total flavones and total anthocyanins were analyzed using spectrophotometer and the composition of the polyphenol extracts was analyzed using HPLC and HPLC-MS. Hierarchical cluster analysis (HCA) was also used to classify the tested results.[Result]Total contents of polyphenols, flavones and anthocyanins of the L. ruthenicum Murr.s were 8.25-87.77 mg GAE·g-1 dry fruit, 18.03-60.44 mg RE·g-1 dry fruit, and 8.21-31.46 mg·g-1 dry fruit, respectively. The samples from Wutumeiren Gomud of Qinghai, Ganhezi of Xinjiang, Baluntai town of Xinjiang, and Helan12-1 of Ningxia had the higher value of total polyphenols, total flavones and total anthocyanins. Through HPLC-ESI-MS analysis, a total of 19 polyphenols were identified in the tested L. ruthenicum Murr, including 7 acylated anthocyanins, and petunidin-3-O-rutinoside (cis-p-coumaroyl)-5-O-glucoside was found to be the main anthocyanin in L. ruthenicum Murr. Twenty polyphenols were found in all kinds of L. ruthenicum Murr, while Petunidin-3-O-rutinoside (cis-p-coumaroyl)-5-O-glucoside was the main composition of the polyphenols in fruits of L. ruthenicum Murr. The polyphenols fingerprints of L. ruthenicum Murr. from 26 different places were similar, excluding a few of samples. The results of HCA according to the contents of polyphenols, flavones and anthocyanins in L. ruthenicum Murr. were clustered into7 kinds. In the sixth and seventh kinds, all the contents of polyphenols, flavones and anthocyanins were higher(57.74-87.77 mg·g-1, 41.52-55.46 mg·g-1, 12.25-28.54 mg·g-1, respectively) in the L. ruthenicum Murr. samples from Zhongqu Mingqin of Gansu, Kashi of Xinjiang, and 12-01 Helan of Ningxia. Especially, the sample from the Ganhezi of Xinjiang would be the good elite material using the index of the contents of polyphenols and anthocyanins. The result of clustering analysis according to HPLC shared peaks’ area of L. ruthenicum Murr. was clustered into five kinds according to the 12 shared peaks’ area, among which the fourth kinds Helan 12-1, Wutumeiren Gomud of Qinghai, Ganhezi of Xinjiang samples’ relative peak area was high, and as the main component, peak 22 relative peak area was between 2 350.84 and 3 092.94 . The samples from Baluntai town of Xinjiang as a separate kinds, its main component peak 22 relative peak area reached up to 3 579.96. [Conclusion]The compositons and total polyphenols contents in fruits of L. ruthenicum Murr. from different places are different, but most of the samples are good resources for development of healthy food which are rich in polyphenols, exceptionally, acylation-petunidin.关键词
黑果枸杞/多酚/花色苷/不同产地/聚类分析Key words
Lycium ruthenicum Murr./polyphenols/anthocyanins/different places/hierarchical cluster analysis (HCA)引用本文复制引用
闫亚美,戴国礼,冉林武,罗青,李晓莺,秦垦,巫鹏举,曾晓雄,曹有龙..不同产地野生黑果枸杞资源果实多酚组成分析[J].中国农业科学,2014,(22):4540-4551,12.基金项目
国家自然科学基金（31360076）、宁夏回族自治区自然科学基金（NZ13101）、宁夏农林科学院院自选项目 （）
