中国农业科学Issue(24)：4904-4914,11.DOI:10.3864/j.issn.0578-1752.2014.24.014
青蒿素对山羊瘤胃发酵和微生物氮素微循环的影响
Effects of Artemisinine on the Rumen Fermentation and Microbial Nitrogen Recycling Rate in Goats
摘要
Abstract
[Objective]Artemisinine is a kind of plant(Artemisia apiacea) extracts which is a potential feed additive to manipulate the rumen fermentation pattern in the future. The main purpose of this study was to search a novel rumen regulating agent and reveal its consequences of manipulation in the rumen. Experiments were conducted to investigate the effect of artemisinine on the rumen fermentation parameters and nitrogen recycling rate between protozoa and bacteria in the rumen.[Method] Four Xuhui goats fitted with rumen cannula were used in the experiment. Animals were randomly assigned into four dietary treatments (adding 0,0.2%, 0.4% and 0.6% artemisinine in diets, respectively) in a 4 × 4 Latin square design. All animals were kept in individual pen and fed withad libitum diet consisted of grinded corn, soybean meal and Chinese ryegrass hay mainly and had free access to clean drinking water. Protozoa engulf bacteria rate and ruminal nitrogen recycling were determined by a novel fluorescence-labeled bacteria technique dyed with 5-([4,6-Dichlorotriazin-2-yl]amino) fluorescein hydrochloride.[Result]The results showed that the extent of pH ranged from 6.85 to 7.16. The addition of artemisinine could decrease the concentration of NH3-N concentration effectively; the consequences of ammonia concentration for 0.4% and 0.6% artemisinine treatments were significantly lower than that of the control group. It was shown that acetate, propionate as well as total volatile fatty acid (TVFA) concentration in the rumen were dramatically improved by adding artemisinine, and the ratio of acetate to propionate for artemisinine treatment groups was significantly lower than that of the control group(P<0.05). With the increasing addition of artemisinine, bacterial protein yields tended to increase, and bacterial protein yield of the highest 0.6% group was very significantly higher than that of the control group(P<0.01). Additionally, compared to the control group, no significant influence was detected in ruminal peptide nitrogen concentration. Additionally, artemisinine led to an increase of bacterial density, and a decrease of protozoal density. It was further observed that, the engulfing rate of rumen protozoa on bacteria was altered by artemisinine, and they were, 320.11 cells/(cell·h), 313.94 cells/(cell·h), 305.00 cells/(cell·h), 278.14 cells/(cell·h) for A, B, C, D, respectively.[Conclusion] It was concluded that artemisinine was more effective at 0.6% DM proportion of diet. It could decrease ammonia concentration and influence on ruminal fermentation pattern accordingly. The addition of artemisinine in diet could drop the total protozoal density and alter protozoa genus profile in the rumen, where the percentage ofEntodiniumwas decreased, and that ofDiplodinium andIsotricha were increased. With adding at 0.6% artemisinine, the rate of microbial protein recycling was increased by decreasing engulfing bacteria rate of protozoa in the rumen of goats and dietary protein efficiency was improved potentially.关键词
青蒿素/瘤胃发酵/微生物氮素循环/山羊Key words
artemisinine/rumen fermentation/microbial nitrogen recycling/goats引用本文复制引用
王洪荣,秦韬,王超..青蒿素对山羊瘤胃发酵和微生物氮素微循环的影响[J].中国农业科学,2014,(24):4904-4914,11.基金项目
国家公益性行业（农业）科研专项（201303144）、国家自然科学基金（30771567,31072051）、江苏高校优势学科建设工程资助项目 （）
