中国农业科学Issue(2)：207-214,8.DOI:10.3864/j.issn.0578-1752.2015.02.01
黄淮麦区部分小麦种质脂肪氧化酶活性分析及等位基因检测
The Allelic Variation of Lipoxygenase Genes in Bread Wheat Cultivars from the Yellow and Huai Wheat Areas of China
摘要
Abstract
[Objective]The objective of this study is to provide useful information for wheat breeding program, a total of 306 wheat cultivars or advanced lines from the Yellow and Huai valleys of China were used to identify LOX (Lipoxygenase) activity and allelic variations of LOX genes in this study.[Method] Spectrophotometer and microplate were used to measure the LOX activity. The molecular marker Xwmc312 on chromosome 1AL and the functional markers LOX16 and LOX18 on chromosome 4BS were employed to identify the polymorphism of the LOX genes in Chinese wheat cultivars or advanced lines surveyed by combinations of PCR (Polymerase Chain Reaction) amplification and agarose and electrophoresis gels. [Result] The phenotyping results showed that the averaged LOX activity was 65.73 AU·min-1·g-1 and the standard deviation (SD) was 13.54 in the wheat cultivars surveyed from the Yellow and Huai valleys, and it ranged from 27.09 to 99.55 AU·min-1·g-1 and its coefficient of variation (CV) was 20.6%. In the wheat cultivars surveyed from the Yellow and Huai valleys, LOX activities of 7 wheat cultivars were less than 40 AU·min-1·g-1 and LOX activities of 8 wheat cultivars exceeded 90 AU·min-1·g-1. These cultivars could provide very important germplasms for improvement of LOX activities of currently popular wheat in wheat breeding program. Genotyping results indicated that three alleles Xwmc312227, Xwmc312235 and Xwmc312247 were found at the QLpx.caas-1AL locus in these wheat varieties with 30.4%, 19.0% and 50.6%, respectively. At TaLOX-B1 locus, two alleles TaLOX-B1a and TaLOX-B1b were identified by the functional markers LOX16 and LOX18 with 28.8% and 71.3%, respectively, in the bread wheat cultivars surveyed. Six allele combinations of LOX genes, i.e., TaLOX-B1a/Xwmc312227, TaLOX-B1a/Xwmc312235, TaLOX-B1a/Xwmc312247, TaLOX-B1b/Xwmc312227, TaLOX-B1b/Xwmc312235 and TaLOX-B1b/Xwmc312247, were found, with 7.2%, 9.5%, 12.1%, 23.2%, 9.5% and 38.6%, respectively, in the bread wheat cultivars surveyed. Analysis of association of LOX alleles and LOX activity indicated that the average of LOX activity of wheat cultivars with Xwmc312235 is significantly higher than those of cultivars with Xwmc312227 and Xwmc312247, and the average of LOX activity of cultivars with TaLOX-B1a is significantly higher than those of cultivars with TaLOX-B1b (P<0.05). Moreover, analysis of association of LOX allele combination and LOX activity showed that the LOX activity (76.803 AU·min-1·g-1) of the cultivars with TaLOX-B1a/Xwmc312235 is significantly higher than those of other five LOX allele combinations, whereas the LOX activity (62.45 AU·min-1·g-1) of the cultivars with TaLOX-B1b/Xwmc312227 genotype is significantly lower than those of other five combinations.[Conclusion]It was concluded that LOX activities of most of the wheat cultivars from the Yellow and Huai valleys were middle type and the wheat cultivars with extremely low (less than 40 AU·min-1·g-1 ) or high (more than 90 AU·min-1·g-1 ) LOX activities were very scarce in the Yellow and Huai wheat regions. Amongst 6 different combinations of LOX genes, wheat cultivars with TaLOX-B1a/Xwmc312235 possessed relatively the highest LOX activity and wheat cultivars with TaLOX-B1b/Xwmc312227 possessed relatively the lowest LOX activity in the Yellow and Huai wheat regions (P<0.05). These results offered important gene germplasms for improvement of LOX activities of bread wheat in wheat breeding program. This study could also provide important information for wheat quality improvement in China.关键词
普通小麦/面粉颜色/脂肪氧化酶/等位变异Key words
bread wheat/flour color/lipoxygenase/allelic variation引用本文复制引用
吴培培,宋双,张福彦,陈锋,崔党群..黄淮麦区部分小麦种质脂肪氧化酶活性分析及等位基因检测[J].中国农业科学,2015,(2):207-214,8.基金项目
国家“973”计划项目（2014CB138105，2014CB160303）、教育部新世纪优秀人才项目（NCET-13-0776）、河南省高校科技创新团队项目 （）
