中国农业科学Issue(3)：426-435,10.DOI:10.3864/j.issn.0578-1752.2015.03.03
豇豆属食用豆类间的远缘杂交
Interspecific Hybridization AmongVigna Species
摘要
Abstract
Objective]The aim of this study was to analyze the crossability of differentVigna species, which will facilitate the distant hybridization breeding ofVigna.[Method] Interspecific crossabilities were investigated among 5 cultivar species of Vigna,V. radiata (mungbean),V. mungo (black gram),V. umbellata (rice bean),V. anguluris (adzuki bean),V. unguiculata (cowpea), and 3 wild relative species of adzuki bean,V. minima,V. nakashiame andV. riukiuensis. Embryo rescue was conducted for 3 interspecific combinations, namely,V. radiata×V. angularis,V. angularis×V. umbellataandV. umbellata×V. angularis, respectively. The pod setting ratio of hybrid and performance of F1 plant were investigated to analyze the crossabilities between food legumes ofVigna genus.[Result] The genetic relationship between different food legumes and the choice of female parent influenced the result of distant hybridization. Except for cowpea cross combinations, hybrid pods were set successfully in the other interspecific crosses. The growth of hybrid pods and seeds showed variations in different combinations. No genetic disorders before fertilization were found from the hybridizations ofV. radiata×V. mungo,V. angularis×V. minima,V. angularis×V. nakashiame, andV. angularis×V. riukiuensis. The hybrid seeds of them germinated normally, and the hybrid plants were fertile, but the fruiting rates were lower. The pod setting ratio was significantly higher whenV. radiatawas selected as female parent in the interspecific crosses betweenV. radiataandV. umbellata. F1 plants were obtained without embryo culture, but they showed complete hybrid sterility. No fertile seeds were obtained even backcross toV. radiataorV. umbellata. Hybrid plants were obtained from cultured embryos for the interspecific crosses ofV. radiata×V. angularisandV. umbellata×V. angularis. The fertility of F1 plants from these two combinations was opposite, sterile in the former and fertile in the latter. The hybrid pods shriveled or fell off, or embryo abortion occurred in all other interspecific crosses.[Conclusion]Using mungbean as the female parent was of benefit to the development of the hybrid pods and seeds when crossed with blackgram, adzuki bean and rice bean, respectively. The embryo abortion occurred when crossed blackgram with adzuki bean and rice bean, respectively. Through the use of embryo culture, the fertile F1 plants could be obtained when using rice bean as the female parent crossed with adzuki bean. The crossability of adzuki bean with its wild relative was better than that of rice bean. All the crosses were failure when using cowpea as one of the parents.关键词
豇豆属/食用豆种/远缘杂交/幼胚拯救Key words
Vigna genus/food legumes/interspecific hybridization/embryo rescue引用本文复制引用
刘长友,范保杰,曹志敏,苏秋竹,王彦,张志肖,程须珍,田静..豇豆属食用豆类间的远缘杂交[J].中国农业科学,2015,(3):426-435,10.基金项目
国家现代农业产业技术体系建设专项（CARS-09）、河北省自然科学基金（C2012301012）、河北省农林科学院青年基金 （）
