中国农业科学Issue(7)：1249-1261,13.DOI:10.3864/j.issn.0578-1752.2015.07.01
海拔差异对水稻雌配子体基因型选择及其后代表型遗传分化的影响
Female Gametophyte Genotype Selection and Its Progeny Phenotypic Genetic Differentiation in Rice at Different Altitude Condition
摘要
Abstract
[Objective] Understanding of rice female gametophyte genotype selection effect and its progeny phenotypic genetic differentiation at different altitude condition could be given a reference for developing breeding method of female gametophyte genotype selection affected by altitude-based change. [Method] Four sites with obvious altitude difference were selected to produce and grow seven F1 female gametophyte genotype selection (FGGS) populations and its 28 F2 progeny segregation (PS) populations generated by crosses between two rice CMS-D1 lines and two cold tolerant japonica landrances, respectively, and then through analysis of the variation of morphological characters of these populations to learn whether the presentations of female gametophyte genotypic frequency variation and its progeny phenotypic genetic differentiation were associated with different altitude backgrounds. [Result] Eight morphological characters of the F1 FGGS populations at the four altitude sites were detected that significant genetic segregation distortions caused by female gametophyte genotype selection which derived from the effect of different altitude conditions, and the influence of two altitudes at 2 200 m and 400 m was greater than that of 1 860 m and 1 250 m, respectively. However, the FGGS populations produced at the altitude of 1 860 m presented the greatest diversity index of morphological characters, and then the index decreased with increase or reduction of the altitude. Comparison of the character variations of these FGGS populations generated at the four altitudes showed a significant difference between at 2 200 m and the other three altitudes. In addition, significant phenotypic genetic differentiation, which resulted from selection of the F1 female gametophyte genotypes affected by different altitude backgrounds, was screened in their all 28 F2 PS populations grown at the four altitudes, and also found bigger segregation while the selection derived from the largely different altitude environments. There were 21 significant differences involved in seven characters of the PS populations derived from two extreme altitudes between the highest altitude of 2 200 m and the lowest altitude of 400 m, and were only six differences involved in three characters from the both middle altitudes 1 860 m and 1 250 m. An essential change was found that the populations selected by the highest altitude had the highest average seed setting rate and poor growth potential, and by the lowest altitude showed the opposite trend against that of the highest, and by the two middle altitudes appeared larger diversified index of morphological characters. [Conclusion] The effect of F1 female gametophyte genotype selection caused by pressure of different growing altitude environments was detected, which is an important factor leading to show phenotypic genetic segregation of its progeny populations, and then to develop new features adapting to corresponding ecological environment. So it is feasible for developing breeding method of female gametophyte genotype selection caused by altitude condition difference.关键词
水稻/雌配子体选择/遗传分化/海拔环境/温度Key words
rice (Oryza sativa L.)/selection of female gametophyte/genetic differentiation/altitude condition/temperature引用本文复制引用
雷伟,谭亚玲,金寿林,谭学林,建成,普世皇,王昌江,刘华萍,孙朝华,苏家秀,李振,徐津..海拔差异对水稻雌配子体基因型选择及其后代表型遗传分化的影响[J].中国农业科学,2015,(7):1249-1261,13.基金项目
国家自然科学基金（31060088，31060058）、云南省教育厅重点基金 （）
