中国农业科学Issue(7)：1277-1287,11.DOI:10.3864/j.issn.0578-1752.2015.07.03
转录因子对木质素生物合成调控的研究进展
Advances in Research of the Regulation of Transcription Factors of Lignin Biosynthesis
摘要
Abstract
Lignin is an important component of secondary cell wall in vascular plants and has important biological functions. Lignin, cellulose and hemicellulose are crosslinked in the cell wall and provide mechanical support for the plant cells and tissues. The hydrophobic property of lignin makes it impermeable to water, which facilitates the long-distance transport of water and nutrients in plant. Lignin and cellulose are natural physical barriers to various pathogens, which improve the defensive ability against biotic and abiotic stresses. While lignin also has some negative effects on the productive practice, e.g., in pulp and paper industry, many chemicals must be used to remove lignin, which increases the cost of pulping and pollution to the environment. High lignin content in the forage decreases the digestibility of livestocks and affect the nutritive value of forages. Higher lignin content also has a negative effect on the fermentation efficiency of biomass energy. Therefore, it is of great significance to improve the lignin degradability by genetic engineering. In higher plants, lignin can be synthesized by phenylpropanoid pathway and specific lignin biosynthesis pathway. Previous research has shown that NAC, MYB and WRKY transcription factors involved in the regulation of lignin biosynthesis pathway. In Arabidopsis, MYB26 can activate the transcription of NST1/NST2; WRKY12 can bind to the promoter region of NST2 and regulate its expression negatively. SND1 (NST3) and NST1 function redundantly in the regulation of secondary wall synthesis in fibers; NST1 and NST2 are redundant in regulating secondary wall thickening in anther walls; VND6 and VND7 mainly involved in xylem vessel differentiation. All these NAC transcription factors can bind to the downstream MYB transcription factors such as MYB83, MYB46 as well as (or) MYB58, MYB63, MYB85 and MYB103 to regulate lignin biosynthesis positively, whereas MYB75 regulates the lignin biosynthesis negatively. Most of the MYBs in this network can bind to the AC elements (I, II and III) in the promoters of the lingin biosynthesis pathway genes and therefore regulate their expression. Some studies also suggested the involvement of bHLH transcription factors in the regulation of lignin biosynthesis pathway. In this paper, advances in research of the regulation of transcription factors on lignin biosynthesis were reviewed, the major regulatory network of lignin biosynthesis in Arabidopsis was produced, some transcription factors related to lignin biosynthesis in other species (e.g. rice, wheat, maize, eucalyptus, pine and populus) were also summarized. With the development of high-throughput sequencing technology, key regulatory transcription factors will be discovered in more species, which will have an important reference to the lignin modification by genetic engineering. <br> Lignin is an important component of secondary cell wall in vascular plants and has important biological functions. Lignin, cellulose and hemicellulose are crosslinked in the cell wall and provide mechanical support for the plant cells and tissues. The hydrophobic property of lignin makes it impermeable to water, which facilitates the long-distance transport of water and nutrients in plant. Lignin and cellulose are natural physical barriers to various pathogens, which improve the defensive ability against biotic and abiotic stresses. While lignin also has some negative effects on the productive practice, e.g., in pulp and paper industry, many chemicals must be used to remove lignin, which increases the cost of pulping and pollution to the environment. High lignin content in the forage decreases the digestibility of livestocks and affect the nutritive value of forages. Higher lignin content also has a negative effect on the fermentation efficiency of biomass energy. Therefore, it is of great significance to improve the lignin degradability by genetic engineering. In higher plants, lignin can be synthesized by phenylpropanoid pathway and specific lignin biosynthesis pathway. Previous research has shown that NAC, MYB and WRKY transcription factors involved in the regulation of lignin biosynthesis pathway. In Arabidopsis, MYB26 can activate the transcription of NST1/NST2; WRKY12 can bind to the promoter region of NST2 and regulate its expression negatively. SND1 (NST3) and NST1 function redundantly in the regulation of secondary wall synthesis in fibers; NST1 and NST2 are redundant in regulating secondary wall thickening in anther walls; VND6 and VND7 mainly involved in xylem vessel differentiation. All these NAC transcription factors can bind to the downstream MYB transcription factors such as MYB83, MYB46 as well as (or) MYB58, MYB63, MYB85 and MYB103 to regulate lignin biosynthesis positively, whereas MYB75 regulates the lignin biosynthesis negatively. Most of the MYBs in this network can bind to the AC elements (I, II and III) in the promoters of the lingin biosynthesis pathway genes and therefore regulate their expression. Some studies also suggested the involvement of bHLH transcription factors in the regulation of lignin biosynthesis pathway. In this paper, advances in research of the regulation of transcription factors on lignin biosynthesis were reviewed, the major regulatory network of lignin biosynthesis in Arabidopsis was produced, some transcription factors related to lignin biosynthesis in other species (e.g. rice, wheat, maize, eucalyptus, pine and populus) were also summarized. With the development of high-throughput sequencing technology, key regulatory transcription factors will be discovered in more species, which will have an important reference to the lignin modification by genetic engineering.关键词
木质素/生物合成/转录因子/调控Key words
lignin/biosynthesis/transcription factor/regulation引用本文复制引用
郭光艳,柏峰,刘伟,秘彩莉..转录因子对木质素生物合成调控的研究进展[J].中国农业科学,2015,(7):1277-1287,11.基金项目
国家自然科学基金（31101129）、河北省自然科学基金 （）
