中国农业科学Issue(8)：1588-1596,9.DOI:10.3864/j.issn.0578-1752.2015.08.13
外源精胺对菜用大豆贮藏冷害及蔗糖代谢的影响
Effect of Exogenous Spermine on Chilling Injury and Sucrose Metabolism of Post-Harvest Vegetable Soybean
摘要
Abstract
[Objective] Mechanism of exogenous spermine in delaying chilling injury and regulating sucrose metabolism of vegetable soybean during cold storage was investigated to provide a theoretical basis for the preservation of post-harvest vegetable soybean.[Method]‘Xindali No. 1’ vegetable soybeans used as the experimental materials were immersed in 0.5, 1 and 2 mmol·L-1 spermine for 20 min, respectively, distilled water as a control, then stored at 1℃ for 10 weeks under the environmental condition of relative humidity of 85%-90%. The chilling resistance related physiological indexes, soluble sugars (sucrose, fructose and glucose) contents and the activities of sucrose metabolism related enzymes were all determined every week.[Result]The chilling injury (CI) symptoms of vegetable soybean aggravated with the extension of storage period, and it appeared during the first two weeks of storage. Spermine treatment not only delayed the increase of CI index and postponed the symptoms until the 3rd week, but also inhibited cell membrane permeability, especially after 5 weeks of storage, both 0.5 and 1 mmol·L-1 spermine treatment groups reached significant levels (P<0.05) compared with the control group. In addition, during cold storage, the membrane lipid peroxidation product, including malondialdehyde (MDA) gradually accumulated in vegetable soybean, low concentration of spermine could reduce the lipid peroxidation and decrease MDA accumulation, while high concentration of spermine had a toxic effect. Compared with the control group, 1 mmol·L-1 spermine significantly improved the activities of antioxidant enzymes (CAT, POD and SOD) of vegetable soybeans during storage. And meanwhile, spermine treatment also significantly (P<0.05) reduced the sucrose loss of vegetable soybean, fructose and glucose contents in spermine-treated vegetable soybean grains were lower than the control group, but the concentration differences among all treatment groups were not significant (P>0.05) during the whole storage period. Through the correlation analysis between soluble sugars contents and sucrose metabolism related enzyme activities in vegetable soybean grains during cold storage, it indicated that sucrose content had a significant negative correlation (P<0.01) with AI activity, but a significant positive correlation (P<0.01) with SPS activity, and no significant correlation with NI and SS activities, which meant AI and SPS played a key role in sucrose degradation of vegetable soybean during cold storage, exogenous spermine effectively inhibited the sucrose loss by regulating the activities of sucrose-metabolizing enzymes in vegetable soybean.[Conclusion] Spermine effectively delayed the chilling injury of vegetable soybean during storage at 1℃, and a dose of 1 mmol·L-1 could be an effective treatment. Sucrose loss might cause the aggravated chilling injury, while spermine maintained higher sucrose content by simultaneously inhibiting the increase of AI activity and the decrease of SPS activity in vegetable soybean during cold storage.关键词
菜用大豆/精胺/贮藏/冷害/蔗糖代谢Key words
vegetable soybean/spermine/storage/chilling injury/sucrose metabolism引用本文复制引用
刘春泉,宋江峰,王远,李大婧..外源精胺对菜用大豆贮藏冷害及蔗糖代谢的影响[J].中国农业科学,2015,(8):1588-1596,9.基金项目
国家自然科学基金（31301534）、南通市重大科技创新专项 （）
