中国农业科学Issue(8)：1609-1615,7.DOI:10.3864/j.issn.0578-1752.2015.08.15
腐胺和脯氨酸对哺乳期仔猪空肠绒毛-隐窝轴上皮细胞的多胺代谢及Wnt信号通路的影响
Impact of Putrescine and Proline on Suckling Piglet Jejunum Villus-crypt Axis Epithelial Polyamine Metabolism and Wnt Signal Pathway
摘要
Abstract
[Objective]The aim of this paper is to study the effects of putrescine and proline on polyamines (putrescine, spermidine and spermine) metabolites, relative mRNA expression levels of Wnt signaling molecules along the villus-crypt axis in the jejunum of sucking piglet.[Method]Eighteen 0-day old newborn crossbred (Landrace × Yorkshire × Duroc) piglets were randomly divided into three groups:control group, putrescine group and proline group. The piglets in control group were administered an equal volume of saline, and that in other two groups received putrescine (5 mg·kg-1 body weight ) and proline (25 mg·kg-1 body weight ), respectively. Piglets were weaned at 14-day old and slaughtered on 3 days postweaning. The jejuna epithelial cells along the crypt-villus axis were isolated by Krebs Henseleit (KH) solution and yield 3“cell fractions”(Fraction 1, 2 and 3). The concentration of polyamine in F1, F2 and F3 was determined by high performance liquid chromatography, and relative mRNA expression of related genes of polyamine metabolic and wnt signaling pathways were measured by real time PCR. [Result] The concentration of putrescine, spermine and spermidine in F1 were significantly increased (P<0.05) in proline group compared to control group, while there was no significant difference in putrescine group (P>0.05). The concentrations of spermine and spermidine in F2 of proline or putrescine groups were significantly higher than that of control group (P<0.05). the concentrations of putrescine, spermine and spermidine in F3 of three group were not changed significantly (P>0.05). The expression of ornithine decarboxylase (ODC) gene in F1 of putrescine group was significantly higher than that of proline group and control group (P<0.05). The expression of arginase in F2 of proline group was significantly higher than that of putrescine group and control group (P<0.05). The expression of SFRP3 in F2 of proline group was significantly higher than that of putrescine group (P<0.05);the expression of sFRP4 in F3 of putrescine group is significantly higher than that of control group and proline group (P<0.05). [Conclusion] Exogenous putrescine and proline increased the concentration of polyamine in differentiated epithelial cells of jejunum in sucking piglet and promoted the polyamine metabolism, however, no statistically significant effect was observed on crypt cell. In addition, exogenous putrescine and proline promote differentiation in the intestinal epithelial cells by Wnt signaling pathways.关键词
腐胺/脯氨酸/空肠/多胺/WntKey words
putrescine/proline/jejunum/polyamine/Wnt引用本文复制引用
王小城,熊霞,杨焕胜,高巍,龚敏,印遇龙..腐胺和脯氨酸对哺乳期仔猪空肠绒毛-隐窝轴上皮细胞的多胺代谢及Wnt信号通路的影响[J].中国农业科学,2015,(8):1609-1615,7.基金项目
国家自然科学基金重点项目（31330075）、国家自然科学基金（31301988）、中国科学院亚热带农业生态研究所青年人才领域前沿项目（ISACX-LYQY-QN-1205）、江西省科技项目 （）
