中国农业科学Issue(9)：1747-1755,9.DOI:10.3864/j.issn.0578-1752.2015.09.08
短时高温暴露对褐飞虱存活和生殖特性的影响
Effects of Short-Term Exposure to High Temperature on the Survival and Fecundity of the Brown Planthopper (Nilaparvata lugens)
摘要
Abstract
Objective]The objective of this study is to clarify the effects of short-term exposure to high temperature on the survival and fecundity of the brown planthopper (Nilaparvata lugensStål).The results will provide a theoretical basis for the prediction of occurrence and treatment of the population ofN. lugens.[Method]Newly-eclosedN. lugensadults (within 24 h of eclosion) were used in the experiments. Five adult pairs were randomly collected and placed in a glass tube in a water bath at 25℃, and the experiments were carried out, at an increasing temperature with a change of 0.1℃·min-1from 25℃ to 33, 35, 37, 39, 39.5 and up until 40℃, and kept at the target temperature for 2 h. The error range was 0.02℃. After treatment, tubes were moved into the phytotron to recover for 1 h, and the survival rate ofN. lugens was examined. Adults rawed in the phytotron were used as the control group.[Result]Within the range of 33-37℃, the survival rate and fecundity ofN. lugenswere not significantly different from the control. Within the range of 39-40℃, the survival rate of N. lugenswere decreased. After exposure to 39.5℃ for 2 h, the survival rate of male and female adults was less than 50%. After exposure to 40℃ for 2 h, the female adult survival rate dropped to 4.5%, and that of the male adults was 6.7%. All of the adults died within 24 h. After exposure to 33-39.5℃, the gradient of the male adult survival curve decreased abruptly than female. The preoviposition period was extended from 3.5-6.3 days with the increasing temperature from 33-39.5℃. The preoviposition period was 3.5 d at 25℃, 4.4 d at 35℃, 4.4 d at 39℃ (P<0.05), and 6.3 d at 39.5℃ (P<0.01), respectively. Furthermore, high temperature had a negative effect on the fecundity. The average fecundity decreased dramatically after exposure to high temperature for 2 h. F1 survival to adulthood was significantly reduced when adults were exposed to 33-39.5℃. The survival rate dropped from 80.4% to 61.8%-75.5% as the temperature increased. Female adults ceased oviposition activities after the exposure to 40℃. The oviposition rhythms and the number of eggs of F1 hatched per day also changed in these heat shock treatments, with prolonged oviposition period and delayed F1 peak hatching time after exposure to 39.5℃.[Conclusion]The survival and reproduction of N. lugens significantly decreased after exposure to temperatures of 39-40℃. When the exposure temperature exceeds 39℃, it has the potential threats to the survival and fecundity ofN. lugens.关键词
褐飞虱/高温暴露/存活/生殖Key words
Nilaparvata lugens/heat stress/survival/fecundity引用本文复制引用
李干金,徐显浩,张海亮,朱敏,崔旭红..短时高温暴露对褐飞虱存活和生殖特性的影响[J].中国农业科学,2015,(9):1747-1755,9.基金项目
国家“973”计划（2010CB126200）、国家公益性行业（农业）科研专项 （）
