中国农业科学Issue(9)：1825-1835,11.DOI:10.3864/j.issn.0578-1752.2015.09.16
锌对5-15周龄鹅免疫、抗氧化功能、金属硫蛋白-Ⅰ基因表达量的影响及变量相关性分析
Effects of Dietary Zinc on Immunity, Antioxidant Capacity and MT-I mRNA Gene Expression Level and Their Factor Correlation Analysis of 5-15 Weeks Old Goose
摘要
Abstract
Objective] This experiment was conducted to determine the required level of zinc in goose feed. The effects of different levels of dietary zinc on immunity, antioxidant capacity and metallothionein (MT-I) mRNA gene expression to 5-15-week-old Qing-Nong-Hui geese were evaluated.[Method] Three hundred and sixty 29-day-old Qing-Nong-Hui geese were randomly selected and divided into six treatment groups with six replicates for each group, and each replicate contained ten geese (5♂+5♀). The content of added dietary zinc in each group was 27.46 (GroupⅠ, control), 77.46 (GroupⅡ), 127.46 (GroupⅢ), 177.46 (GroupⅣ), 227.46 (GroupⅤ), and 277.46 (GroupⅥ) mg·kg-1, respectively. The geese were fed for 11 weeks.[Result]The results were as follows: Index of thymus, spleen and bursa of fabricius showed an increase and downward trend with the effect of zinc level in feed. The thymus index obtained the highest (P<0.05) level when the added zinc in feed was 127.46 mg·kg-1, while the spleen index and the fabricius bursa index reached the highest level (P<0.05) when the added zinc in feed was 77.46 mg·kg-1. The index of immune organs decreased as long as the dietary zinc in feed was out of the range of 77.46-127.46 mg·kg-1. The antibody titer reached the highest (P<0.05) after the geese were immunized by goose paramyxovirus vaccine for 14 days when the zinc level in feed was 157.41 mg·kg-1. The activity level of total antioxidant capacity (T-AOC) for the goose serum and liver reached the highest (P<0.01) when the zinc level in feed was 153.84 mg·kg-1. The activity level of both catalase (CAT) and glutathione peroxidase (GSH-Px) for the serum and liver of goose was the highest (P<0.01) when the zinc level in feed was 148.33 mg·kg-1. The activity level of superoxide dismutase (CuZn-SOD) was the highest (P<0.01) in the goose’s serum and liver when the zinc level in feed was 148.33 mg·kg-1. On the other hand, when the zinc level in feed was 177.46 mg·kg-1, the malonaldehyde (MDA) in the serum and liver had the lowest level (P<0.05); With the increase of the zinc level in feed, the expression quantity of MT-I mRNA was significantly increased (P<0.05). And when the zinc level in the feed was 177.46 mg·kg-1, the expression quantity of MT-I mRNA of liver was the highest. It was highly significant that the expression quantity of MT-I mRNA in goose liver was positively correlated with the activity level of T-AOC (r= 0.94,P<0.01) and CuZn-SOD (r= 0.97,P<0.01). The MT-I mRNA expression level in goose liver was also positively correlated with the activity level of CAT (r=0.85,P<0.05), GSH-Px (r=0.89,P<0.05), and negatively correlated with MDA (r=-0.70,P>0.05). The expression level of MT-I mRNA in goose liver was significantly positively correlated with activity levels of T-AOC (r=0.99, P<0.01), CAT (r=0.92,P<0.01) and CuZn-SOD (r=0.94,P<0.01) in serum. It was also positively correlated with GSH-Px (r=0.81,P>0.05) and negatively correlated with MDA (r=-0.39, P>0.05).[Conclusion] Appropriate additional level of dietary zinc promoted the immune organs development and antioxidant capacity of goose. Dietary zinc also intervened the MT-I mRNA expression level, which was closely related with the body’s antioxidant capacity. Feed zinc levels in the 77-150 mg·kg-1 range can keep the body in high immunity and antioxidant capacity level of the 5-15 weeks old geese.关键词
锌/鹅/免疫/抗氧化能力/金属硫蛋白-Ⅰ表达量/相关性Key words
zinc/geese/immune/antioxidant capacity/MT-I mRNA gene/correlation analysis引用本文复制引用
王宝维,陈苗璐,王秉翰,张名爱,葛文华,程凡升,岳斌..锌对5-15周龄鹅免疫、抗氧化功能、金属硫蛋白-Ⅰ基因表达量的影响及变量相关性分析[J].中国农业科学,2015,(9):1825-1835,11.基金项目
国家水禽产业技术体系专项基金 （）
