中国普通外科杂志Issue(10)：1367-1372,6.DOI:10.7659/j.issn.1005-6947.2014.10.012
miRNA-21、miRNA-135b、miRNA-141在结肠癌中的表达及其意义
Expressions of miRNA-21, miRNA-135b and miR-141 in colon cancer and the significance
姜训圳 1赵科 1何向辉1
作者信息
- 1. 天津医科大学总医院 普通外科，天津 300052
- 折叠
摘要
Abstract
Objective: To investigate the expressions of miRNA-21, miRNA-135b and miRNA-141 in colon cancer and their signiifcance. <br> Methods: The expressions of the three miRNAs in colon cancer tissues and their adjacent normal colonic mucosal tissues, as well as in the plasma of colon cancer patients and healthy controls were determined by lfuorescent real-time quantitative PCR. hTe relations of the three miRNAs with clinicopathologic factors of colon cancer patients were analyzed. <br> Results: hTe expression levels of the three miRNAs in colon cancer tissue were all signiifcantly higher than those in adjacent colonic mucosal tissue, and in plasma of colon cancer patients were all signiifcantly higher than those in plasma of healthy controls (allP<0.05). In colon cancer patients, the miR-21 expression level was signiifcantly related to TNM stage and invasion depth, the miR-135b expression level was signiifcantly associated with TNM stage and lymph node metastasis, and the miR-141 expression level was significantly linked to TNM stage, invasion depth and lymph node metastasis (allP<0.05). hTe miRNA21 showed no obvious correlation between its expression level in colon cancer tissue and plasma of the colon cancer patients (r=0.459,P=0.072), but either miRNA135b or miRNA-141 had a positive correlation between its expression level in colon cancer tissue and plasma of the colon cancer patients (r=0.686,P=0.042;r=0.742,P=0.026). <br> Conclusion: hTe expressions of miRNA-21, miRNA-135b and miRNA-141 are all up-regulated in colon cancer patients, and they may be closely related to the recurrence and development of this cancer, as well as could be potential tumor markers or therapeutic targets.关键词
结肠肿瘤/微RNAs/肿瘤标记,生物学Key words
Colonic Neoplasms/MicroRNAs/Tumor Markers, Biological分类
医药卫生引用本文复制引用
姜训圳,赵科,何向辉..miRNA-21、miRNA-135b、miRNA-141在结肠癌中的表达及其意义[J].中国普通外科杂志,2014,(10):1367-1372,6.
