中国普通外科杂志Issue(10)：1379-1384,6.DOI:10.7659/j.issn.1005-6947.2014.10.014
整合素受体拮抗剂西仑吉肽对大鼠肠纤维化的治疗作用
Therapeutic effect of integrin receptor antagonist cilengitide on intestinal fibrosis in rats
摘要
Abstract
Objective: To investigate the therapeutic effect of integrin receptor antagonist cilengitide on intestinal ifbrosis in rats. <br> Methods: Twenty-one SD rats were equally randomized into control group, model group and cilengitide treatment group, Intestinal fibrosis model was induced in rats in the latter two groups by continuous intracolonically injection with increasing doses of trinitrobenzene sulfonic acid (TNBS) in 45% ethanol solution for 6 weeks, and rats in cilengitide treatment group simultaneously received daily intraperitoneal injection of cilengitide, while those in the other two groups were given normal saline of the same volume in the same administration manner. During the experimental period, the general conditions were observed and the changes in body weight were recorded in each group of rats. hTe rat colonic tissue specimens were harvested atfer 6 weeks, the intestinal inlfammation and collagen deposition were examined and the levels of TGF-β1, collagen type Iα1 mRNA and collagen type I protein in colonic tissues were measured. <br> Results: Except for control group, rats in the other two groups showed poor general conditions and an initial decrease and subsequent increase in body weight, but the general conditions in rats in cilengitide treatment group were better than those in model group, and the amplitude of later body weight gain in rats in cilengitide treatment group was signiifcantly greater than that in model group (P<0.05); rats in these two groups had evident chronic inlfammation and deposition of collagen ifbers in the colonic tissues, but the histopathological score and collagen ifber content in cilengitide treatment group were signiifcantly lower than those in model group (bothP<0.05). Compared with control group, the colonic levels of total TGF-β1 and activated TGF-β1, and levels of collagen type Iα1 mRNA and collagen type I protein were signiifcantly increased in rats in the other two groups, but the levels of activated TGF-β1, collagen type Iα1 mRNA and collagen type I protein in cilengitide treatment group were signiifcantly lower than those in model group (allP<0.05). <br> Conclusion: Cilengitide can attenuate intestinal ifbrosis through inhibiting TGF-β1 activation resulting from its integrin receptor antagonizing effect.关键词
结肠炎/纤维化/西仑吉肽/转化生长因子β1Key words
Colitis/Fibrosis/Cilengitide/Transforming Growth Factor β1分类
医药卫生引用本文复制引用
陶庆松,陈尧,王宝偲,郑宇..整合素受体拮抗剂西仑吉肽对大鼠肠纤维化的治疗作用[J].中国普通外科杂志,2014,(10):1379-1384,6.基金项目
国家自然科学基金资助项目（81000153）；江苏省自然科学基金资助项目（BK2010415）。 （）
