中国普通外科杂志Issue(1)：45-51,7.DOI:10.3978/j.issn.1005-6947.2015.01.009
载VEGF siRNA环五肽RGD偶联纳米金颗粒的基本特性及在射频消融中的应用
General properties and application of VEGF siRNA-loaded RGD-conjugated gold nanoparticles in radiofrequency ablation
摘要
Abstract
Objective:To investigate the general properties of the complexes of VEGF siRNA-loaded RGD-conjugated gold nanoparticles (GNPs) (GNPs-TyrRGD-VEGFsiRNA) and their impact on necrotic effect of radiofrequency ablation (RFA) in the liver. <br> Methods:GNPs-TyrRGD-VEGFsiRNA complexes were synthetized by chemical and electrostatic adsorption methods and the discreteness of the complexes was examined by electron microscope. hTe stability of the VEGF siRNA in the complexes was tested by gel electrophoresis, the cytotoxicity of complexes to normal hepatic cells <br> was determined by CCK-8 assay, the binding capacities of the complexes to normal hepatic cells and liver cancer cells after co-incubation were analyzed by using electron microscopy, and the impact of the complexes on the necrotic effect of RFA were observed in the in-vitro pig liver. <br> Results: hTe complexes showed a higher discreteness than the naked GNPs and the content of VEGF siRNA in the complexes was approximately equal to the initially loaded content. Both the complexes and naked GNPs exhibited almost no cytotoxicity to the normal hepatic cells at a concentration below 30%and the binding ability of the complexes to the liver cancer cells was signiifcantly greater than that to the normal hepatic cells (P<0.05), but the naked GNPs showed no difference between binding ability to the two types of cells (P>0.05). hTe lesion diameter induced by RFA with the complexes injected through radiofrequency needle was signiifcantly larger than that in control group with saline injection [(2.19±0.24) cm vs. (1.71±0.14) cm, P<0.05]. <br> Conclusion:GNPs-TryRGD-VEGFsiRNA complexes can integrate targeted concentration and targeted therapy as a whole, and may also be helpful for enhancing the effcacy of liver cancer RFA treatment.关键词
肝肿瘤/纳米复合物/药物载体/导管消融术Key words
Liver Neoplasms/Nanocomposites/Drug Carriers/Catheter Ablation分类
医药卫生引用本文复制引用
熊青,邹孟达,邓青松,李晓武,马宽生,别平..载VEGF siRNA环五肽RGD偶联纳米金颗粒的基本特性及在射频消融中的应用[J].中国普通外科杂志,2015,(1):45-51,7.基金项目
国家自然科学基金资助项目（81272688）。 （）
