中国普通外科杂志Issue(3)：363-369,7.DOI:10.3978/j.issn.1005-6947.2015.03.011
下调NDRG1表达对胰腺癌细胞增殖与凋亡的影响
Influence of down-regulation of NDRG1 expression on proliferation and apoptosis in pancreatic cancer cells
摘要
Abstract
Objective:To investigate NDRG1 expression and its relation with MMP-7 in pancreatic cancer cells. <br> Methods:The protein and mRNA expressions of NDRG1 and MMP-7 in 4 different pancreatic cancer cell lines (PANC-1, BXPC-3, CAPAN-2 and SW1990) were determined. hTe interference plasmids siNDRG1 were transfected into PANC-1 cells atfer their construction, and then the protein and mRNA expressions of NDRG1 and MMP-7, cell proliferation and apoptosis atfer transfection were determined. <br> Results:All the 4 cell lines presented either the protein or mRNA expressions of both NDRG1 and MMP-7, and their expression levels were increased with decrease of the degree of cell differentiation (all P<0.05). In PANC-1 after siNDRG1 trasfection, the protein and mRNA expressions of both NDRG1 and MMP-7 were reduced, proliferation was suppressed, and apoptosis rate was increased signiifcantly (all P<0.05). <br> Conclusion:NDRG1 expression is closely related to the degree of differentiation of pancreatic cancer cells, and may probably promote cell growth through up-regulating MMP-7 expression.关键词
胰腺肿瘤/N-myc下游调节基因1/细胞分化Key words
Pancreatic Neoplasms/N-myc Downstream Regulated Gene 1/Cell Differentiation分类
医药卫生引用本文复制引用
张小薄,高峰,谭晓冬,周磊,王怀涛,石刚..下调NDRG1表达对胰腺癌细胞增殖与凋亡的影响[J].中国普通外科杂志,2015,(3):363-369,7.基金项目
国家自然科学基金资助项目（30973501）。 （）
