中国全科医学Issue(14)：1617-1620,4.DOI:10.3969/j.issn.1007-9572.2014.14.013
散发性结直肠癌组织p33 ING1 b启动子甲基化情况及其意义
Promoter Methylation of p33ING1b in Colorectal Cancer
摘要
Abstract
Objective To investigate the expression of promoter methylation of p33ING1b in sporadic colorectal cancer (SCRC),to analyze the its mechanism. Methods Samples of cancer and adjacent para - carcinoma tissues were collected in 63 SCRC patients hospitalized in this hospital from October 2008 to March 2012,and the hemorrhoids mucosa tissues collected in 13 patients who had procedure for prolapse and hemorrhoids(PPH). Promoter methylation of p33ING1b was determined by Nested - methylation specific PCR( nMSP method),expression of p33ING1b mRNA by RT - PCR. 5 - aza - 2 - deoxycytidine (5 - aza - dC)was used to do intervene human colorectal cancer cell line DLD -1,Caco -2 demethylation. Results The positive rate of promoter methylation of p33ING1b was 82. 5% (52/63) in cancer tissues,34. 2% (27/63) in para - carcinoma tissues. No promoter methylation of p33ING1 was detected in hemorrhoids mucosa tissues. The positive rate of promoter methylation of p33ING1 was higher in cancer tissues than in para - carcinoma and hemorrhoids mucosa tissues(χ 2 =21. 21,P ﹤0. 001;χ 2 =33. 98,P ﹤0. 001). There was no significant difference in positive rate of promoter methylation of p33ING1b between patients of different genders,ages,with different cancer locations,degrees of differentiation,lymph node metastasis,distant metastasis (P ﹥ 0. 05),there was between patients with different Dukes stages( P ﹤ 0. 05). The relative transcript level of p33ING1b mRNA was(0. 62 ±0. 15)in cancer tissues of promoter methylation of p33ING1b,lower than in non - methylation cancer tissues(0. 75 ±0. 17)(t =2. 553,P ﹤0. 05). After 5 - aza - dC intervention,promoter methylation of p33ING1b of human colorectal cancer cell line DLD -1,Caco -2 was reversed,and relative transcript level of p33ING1b mRNA was higher the genesis and development of colorectal cancer by inhibiting the expression of p33ING1b mRNA.关键词
结直肠肿瘤/p33ING1b/甲基化/脱氧胞苷Key words
Colorectal neoplasms/p33ING1b/Methylation/Deoxycytidine分类
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张磊昌,何纯钢,曹云飞,钟武,钟世彪,龙军先,黄永红,陈利生..散发性结直肠癌组织p33 ING1 b启动子甲基化情况及其意义[J].中国全科医学,2014,(14):1617-1620,4.基金项目
国家自然科学基金资助项目（30760246）；广西教育厅面上项目 （）
