中国全科医学Issue(18)：2103-2108,6.DOI:10.3969/j.issn.1007-9572.2014.18.012
慢性肾衰竭模型中透明质酸和CD44对骨髓间充质干细胞归巢及修复作用的影响
Effect of Hyaluronic Acid and CD44 on Homing and Improving Renal Function of Mesenchymal Stem Cells in A Rat Chronic Renal Failure Model
摘要
Abstract
Objective To explore the effect of hyaluronic acid( HA)and CD44 on the homing and improving renal function of Mesenchymal Stem Cells( MSC)in a rat chronic renal failure model. Methods 32 grade clean adult male SD rats were randomly divided into 4 groups:sham operation group,renal failure group,MSC group,MSC/anti -CD44 group,8 in each group. Establishment of rat remnant kidney model:after one week adaptive breeding,all cut right kidney first,then cut 2/3 left kidney 1 week later. But in sham operation group,after anesthesia,rats were only given renal capsule stripping. 8 weeks af-ter model establishment,1 ml MSC(1 × 107/ml),1 ml PBS,1 ml MSC(1 × 107/ml)and 1 ml MSC(1 × 107/ml)incuba-ted with OX50(anti CD44 antibody)were injected via tail vein in every group respectively. After treatment of 4 weeks,all rats were killed and blood sample and kidney tissue were collected. Serum Cr and BUN were tested. Pathological changes of kidney were assessed by PAS and Masson stain. Glomerulosclerosis index and renal tubular mesenchymal damage index were calculated by semi-quantitative method. Tracing MSC using immunofluoresent assay. Double fluorescence immunostain of MSC and HA was used in kidney sections of rat. Expression of HA was assayed by immunohistochemical staining and western blot. Results Com-pared with sham operation group,renal failure group displayed significantly elevated Cr,BUN,glomerulosclerosis index and renal tubular mesenchymal damage index,and the difference was significant(P<0. 05). The results of PAS and Masson staining in-dicated that compared to sham operation group major pathological changes in residule kidney tissues were glomerular hypertrophy, cloudy swelling of renal epithelial cells,focal mesangial cell proliferation,widened mesangial matrix,focal glassy degeneration of glomerular capillary,renal interstitial inflammatory cell infiltration,focal interstitial fibrosis. MSC and MSC/anti-CD44 treat-ments could alleviate the above pathological changes in MSC group and MSC/anti-CD44 group(P<0. 05). After treatment of 4 weeks,there was no MSC homing in sham operation group,increased MSC homing was detected in residule kidney tissues after MSC administration,whereas the number of MSC homing was decreased in MSC/anti-CD44 group. There was no expression HA in sham operation group,but HA was significantly observed in injured cortical renal tissue. MSC was likely home to the area con-tained abundant HA in renal tissue. Conclusion Interaction between CD44 and HA has the potential to influnce MSCs homing to remnant kidney. This impact might supress MSC homing,but the ability of MSC in recovery of renal function was unchanged.关键词
肾功能衰竭,慢性/骨髓间充质干细胞/透明质酸/CD44/归巢Key words
Kidney failure,Chronic/Mesenchymal stem cell/Hyaluronic acid/CD44/Homing分类
医药卫生引用本文复制引用
边晓慧,何平,孙广萍,孙立,李德天,冯江敏..慢性肾衰竭模型中透明质酸和CD44对骨髓间充质干细胞归巢及修复作用的影响[J].中国全科医学,2014,(18):2103-2108,6.基金项目
辽宁省自然科学基金 （）
