中国全科医学Issue(24)：2826-2830,5.DOI:10.3969/j.issn.1007-9572.2014.24.011
三氧化二砷联合热疗对食管癌细胞株EC-1放疗的增敏作用研究
Radiosensitizing Effect of Aresenic Trioxide in Conjunction with Hyperthermia on EC-1 Human Esophageal Squamous Carcinoma Cells
摘要
Abstract
Objective To investigate the radiosensitizing effect and underlying mechanism of arsenic trioxide ( As2 O3 )in combination with hyperthermia on human esophageal squamous carcinoma cell line EC-1. Methods There were four groups as the irradiation group ( R ), the hyperthermia with irradiation group ( HR ), the As2 O3 with irradiation group (AR)and the As2O3 with hyperthermia and irradiation group(AHR). Clonogenic radiation survival assays on EC-1 cell line after treatments with As2 O3 and hyperthermia with or without radiation were adopted. Cell survival curve was gained using single-hit multitarget model. Cell cycles and the cell apoptosis rate of the 4 different groups were tested by flow cytometric analysis (FCM). All experiments were repeated 3 times. Results According to the clonogenic radiation survival assay,the radiobiologi-cal parameters D0 ,Dq and N in the R,HR,AR and AHR groups was 2. 192 Gy,2. 070 Gy,1. 705 Gy,1. 272 Gy;0. 461 Gy,0. 241 Gy,0. 134 Gy,0. 042 Gy;1. 625,1. 308,1. 192,1. 079. The SERD0 and SERDq in the HR,AR and AHR groups was 1. 059,1. 286,1. 723;1. 917,3. 474,11. 000. The FCM showed that cells in G0/G1 phases and S phases were significantly lower in HR,AR and AHR groups than R group(P<0. 05),while cells in G2/M phase were significantly higher in HR,AR and AHR groups than R group(P<0. 05). Cells in G0/G1 phases were lower in AR group than HR group(P<0. 05). Cell cycles in AHR group was obvious different compared to AR and HR groups(P<0. 05). Cell apoptosis was en-hanced in other 3 groups compared to R group(P<0. 05). Among the 3 groups,cell apoptosis in AHR group was higher than HR and AR groups(P<0. 05). Conclusion Both As2O3 and hyperthermia can enhance the radiosensitivity in EC-1,and combination of them could enhance this effect more significantly. The change of EC-1 cell cycle,including the increase of G2/M and decrease of G0/G1 and S,may be responsible for the radiosensitivity effect. Meanwhile,the induction of apoptosis which is benefit to the killing effect of radiotherapy on EC-l cell was also involved in this process.关键词
食管肿瘤/三氧化二砷/高温,诱发/放射疗法/辐射增敏药Key words
Esophageal neoplasms/Arsenic trioxide/Hyperthermia,induced/Radiotherapy/Radiation-sensiti-zing agents分类
医药卫生引用本文复制引用
谷见法,冯沛贝,路平..三氧化二砷联合热疗对食管癌细胞株EC-1放疗的增敏作用研究[J].中国全科医学,2014,(24):2826-2830,5.基金项目
河南省科技厅资助项目 （）
