中国全科医学Issue(27)：3264-3268,5.DOI:10.3969/j.issn.1007-9572.2014.27.028
通心络胶囊对糖尿病视网膜病变KK/Upj-Ay小鼠血液及视网膜炎性因子表达的影响
Effect of Tongxinluo℅apsule on Inflammatory℅ytokines Expression of Blood and Retina in KK/Upj-Ay Mice with Dia-betic Retinopathy
摘要
Abstract
Objective ToinvestigatetheeffectofTongxinluocapsuleoninflammatorycytokinesexpressionofblood andretinainKK/Upj-Aymicewithdiabeticretinopathy(DR).Methods 40spontaneoustype2diabeticKK/Upj-Aymice (12 weeks of age,male)and 10 C57BL/6 mice(12 weeks of age,male)were selected as study subjects. According to fasting blood-glucose( FBG)level and random number table,KK/Upj-Ay mice were divided into model group,Tongxinluo low dose group(1 g/kg),Tongxinluo middle dose group(2 g/kg)and Tongxinluo high dose group(4 g/kg),10 mice in each group. Mice in Tongxinluo group were given Tongxinluo capsule intragastrically,once a day,for 12 weeks. The same volume of distilled water was given to the mice in control group and model group,once a day,for 12 weeks. 12 weeks after treatment,the function of blood retinal barrier was determined. Blood levels of inflammatory cytokines, such as tumor necrosis factor alpha (TNF-α),intercellular adhesion molecule 1(ICAM-1),interleukin 6(IL-6)and interleukin 1 beta(IL-1β)were de-termined by FCM. The mRNA and protein expression levels of inflammatory cytokines in retina were determined by real-time PCR andWesternblotting,respectively.Results 12weeksaftertreatment,retinaevansblue(EB)contentofmiceinmodelgroup were significantly higher than that in control group(P<0. 05). Blood levels of TNF-α,ICAM-1,IL-6 and IL-1β of mice in model group were significantly higher than those in control group(P<0. 05). The retina mRNA and protein expression levels of TNF-α,ICAM-1,IL-6 and IL-1β of mice in model group were higher than those in control group(P<0. 05).The retina EB content of mice in Tongxinluo middle dose group and Tongxinluo high dose group were significantly lower than that in model group,respectively(P<0. 01). Blood levels of TNF-α,IL-1β and ICAM-1 of mice in Tongxinluo middle dose group and Tongxinluo high dose group were significantly lower than those in model group,respectively(P<0. 05). Blood IL-6 levels of mice in Tongxinluo low,middle,high dose groups were significantly lower than that in model group,respectively( P<0. 05). The retina mRNA and protein expression levels of TNF-α,ICAM-1,IL-6 and IL-1β of mice in Tongxinluo low, middleandhighdosegroupsweresignificantlylowerthanthoseinmodelgroup(P<0.01).℅onclusion Tongxinluocapsule can improve blood retinal barrier function of KK/Upj-Ay mice,and inhibit the inflammatory cytokines expression of whole body and retina.关键词
通心络胶囊/糖尿病视网膜病变/肿瘤坏死因子α/细胞黏附分子1/白介素6/白介素1βKey words
Tongxinluo capsule/Diabetic retinopathy/Tumor necrosis factor-alpha/Cell adhesion molecules-1/Interleukin-6/Interleukin-1beta分类
医药卫生引用本文复制引用
王超,邢邯英,王杏,刘敏,张哲..通心络胶囊对糖尿病视网膜病变KK/Upj-Ay小鼠血液及视网膜炎性因子表达的影响[J].中国全科医学,2014,(27):3264-3268,5.基金项目
国家重点基础研究发展计划（973计划）项目 （）
