中国全科医学Issue(30)：3575-3578,3584,5.DOI:10.3969/j.issn.1007-9572.2014.30.012
透明质酸对体外培养的大骨节病和骨关节炎软骨细胞透明质酸合成酶2 mRNA表达的影响
Effect of Hyluronic Acid on the Expression of HAS2's mRNA in Chondrocytes of Kashin-beck Disease and Osteoarthri-tis Cultured in Vitro
摘要
Abstract
Objective To investigate the effect of hyluronic acid(HA)on the expression of HAS2's mRNA in chon-drocytes of Kashin-beck disease( KBD)and osteoarthritis( OA)cultured in vitro. Methods A total of 6 patients who were di-agnosed with KBD in Shanxi Provincial Institute for Endemic Disease from August 2006 to July 2008 , were selected as KBD group,the loose bodies and articular cartilage of KBD patients were removed through surgery. A total of 6 patients who were diag-nosed with OA in Xi'an City Red Cross Hospital,were selected as OA group,the knee articular cartilage of OA patients were re-moved through total knee arthroplasty. At the same time,amputees and the dead(6 cases)due to accidents such as traffic acci-dent,were selected as control group. Cells from 3 groups were treated with different doses of HA(0 μg/ml,100 μg/ml and 500μg/ml),the effect of HA on the expression of HAS2's mRNA in 3 groups of chondrocytes was detected by RT-PCR. Results Before treatment,there were significant differences in the expression levels of HAS2's mRNA among 3 groups of chondrocytes(P<0. 05). The HAS2's mRNA expression level in OA group was significantly lower than that in control group(P<0. 05). After treatment,there was no significant difference in the expression levels of HAS2's mRNA among different dose groups(P>0. 05). Conclusion The capacity of HA synthesis decreases in chondrocytes of KBD and OA. The exogenous HA can enhance chondro-cytes' capacity of HA synthesis,which provides the theory foundation for treating KBD and OA with intraarticular injection of HA.关键词
大骨节病/骨关节炎/透明质酸/软骨细胞/体外研究/透明质酸合成酶2Key words
Kashin-beck disease/Osteoarthritis/Hyaluronic acid/Chondrocytes/In vitro/HAS2分类
医药卫生引用本文复制引用
高宗强,郭雄,陈君长,段琛,马玮娟,刘瑞宇,顾其胜..透明质酸对体外培养的大骨节病和骨关节炎软骨细胞透明质酸合成酶2 mRNA表达的影响[J].中国全科医学,2014,(30):3575-3578,3584,5.基金项目
国家自然科学基金重点项目（30630058）；科技部国际合作重点专项基金（2006DFA33610）；陕西省社发公关基金 （）
