摘要
Abstract
Objective To investigate the dynamic expression of Prolyl Hydroxulase 3(PHD3)and Hypoxia - induci-bla Facctor - 1α(HIF - 1α)in non - small cell lung cancer(NSCLC). And to explore its clinical significance in the pathogen-esis of NSCLC. Methods Eighty - nine cases of NSCLC tumor and adjacent normal lung tissue samples were collected from July 2010 to April 2012 in Affiliated Hospital of Hebei University. Taking the NSCLC tissue,adjacent normal lung tissue(from the tumor edge > 5 cm)specimens were test. 51 cases with lymph node metastasis(lymph node metastasis group),38 cases with-out lymph node metastasis(without lymph node metastasis group);tumor staging(TNM):26 cases of stage Ⅰ,34 cases of stage Ⅱ,29 cases of stageⅢ. RT - PCR was used to determine the expression of mRNA,Western blotting to determine the ex-pression of proteins. Results PHD3 mRNA was higher in tumor tissues than in adjacent normal lung tissues〔(17. 8 ± 2. 9) vs. (7. 1 ± 1. 3 )〕,the difference was statistically significant(t = 31. 763,P < 0. 05);PHD3 protein declined in tumor tis-sues〔(13. 0 ± 2. 8)vs. (20. 7 ± 4. 2)〕,the difference was statistically significant(t = 14. 391,P < 0. 05). The relative expression of mRNA and protein in lung cancer tissue and NSCLC of different clinical stages of PHD3 weight,the difference was statistically significant( P < 0. 05);with the increase of TNM stage,PHD3 mRNA relative expression quantity was higher, while PHD3 protein expression quantity was relatively low(P < 0. 05). Adjacent normal lung tissue and lymph node metastasis group mRNA and protein in the relative expression of PHD3 comparison,the difference was statistically significant(P < 0. 05);and the lymph node metastasis group PHD3 mRNA relative expression quantity was higher than the adjacent normal lung tissue and without lymph node metastasis group,PHD3 protein expression levels lower( P < 0. 05). The expression of HIF - 1α mRNA was significantly higher in tumor tissues than in adjacent tissues〔(17. 2 ± 2. 9)vs. (6. 5 ± 1. 2 ),t = 32. 163,P < 0. 05〕, and HIF - 1α protein higher in tumor tissues than in adjacent tissues〔(20. 9 ± 3. 8) vs. (9. 2 ± 1. 8 ),t = 26. 251,P <0. 05〕. The relative expression of mRNA and protein in lung cancer tissue and NSCLC of different clinical stages of HIF - 1αquantity comparison,the difference was statistically significant(P < 0. 05);with the increase of TNM stage,HIF - 1α mRNA, HIF - 1α protein relative expression quantity was higher(P < 0. 05). Adjacent normal lung tissue and lymph node metastasis group mRNA and protein HIF - 1α relative expression of the comparison,the difference was statistically significant(P < 0. 05);and the lymph node metastasis group mRNA and protein HIF - 1α relative expression quantity was higher than the adjacent normal lung tissue and the group without lymph node metastasis(P < 0. 05). Linear correlation analysis showed that PHD3 protein nega-tively(r = - 0. 746,P < 0. 05),that PHD3 mRNA was positively correlated with HIF - 1α protein(r = 0. 792,P < 0. 05). Conclusion PHD3 may down - regulate HIF - 1α expression during the development of NSCLC,which can be a target for NSCLC therapy.关键词
脯氨酰羟化酶/低氧诱导因子 1α/癌,非小细胞肺/逆转录聚合酶链反应/免疫印迹法Key words
Prolyl hydroxylases/Hypoxia - inducible factor 1α/Carcinoma,non - small - cell lung/Reverse tran-scriptase polymerase chain reaction/Immunoblotting分类
医药卫生
