中国全科医学Issue(3)：278-282,5.DOI:10.3969/j.issn.1007-9572.2015.03.010
细胞凋亡在维生素 K2抑制高磷诱导的大鼠血管平滑肌细胞钙化中的作用
Ettects ot Cell Apoptosis on High-phosphorus-induced Rat Vascular Smooth Muscle Cells Calcitication Inhibited by Vitamin K2
摘要
Abstract
Objective To explore the effects of vitamin K2 on high - phosphorus - induced calcification of rat vascular smooth muscle cells(VSMCs),and the role played by cell apoptosis. Methods A total of 6 healthy female SD rats aged 8 - 10 weeks were selected,VSMCs from rat thoracic aorta were cultured in vitro,and identified by immunocytochemistry. VSMCs were randomly divided into 5 groups by random number table method:control group,high phosphorus group,vitamin K2 group 1(10μmol/ L),vitamin K2 group 2(25 μmol/ L),vitamin K2 group 3(50 μmol/ L). The cell calcium content,the expression of Axl and Bcl - 2 mRNA,and the cell apoptosis rate were measured. Results There was significant difference in calcium content of VSMCs among 5 groups of rats(P ﹤ 0. 05). The VSMCs calcium level in high phosphorus group,vitamin K2 group 1,vitamin K2 group 2,vitamin K2 group 3 was significantly higher than that in control group,respectively( P ﹤ 0. 05). The VSMCs calcium level in vitamin K2 group 1,vitamin K2 group 2,vitamin K2 group 3 was significantly lower than that in high phosphorus group,respectively(P ﹤ 0. 05). The VSMCs calcium level in vitamin K2 group 2,vitamin K2 group 3 was significantly lower than that in vitamin K2 group 1,respectively(P ﹤ 0. 05). The VSMCs calcium level in vitamin K2 group 3 was significantly lower than that in vitamin K2 group 2( P ﹤ 0. 05). There was significant difference in the expression level of Axl mRNA in VSMCs among 5 groups of rats(P ﹤ 0. 05). The expression level of VSMCs Axl mRNA in high phosphorus group and in vitamin K2 group 1 was significantly lower than that in control group,respectively( P ﹤ 0. 05). The expression level of VSMCs Axl mRNA in vitamin K2 group 3 was significantly higher than that in control group(P ﹤ 0. 05). The expression level of VSMCs Axl mRNA in vitamin K2 group 2 and in vitamin K2 group 3 was significantly higher than that in high phosphorus group,respectively (P ﹤ 0. 05). There was no significant difference in the expression level of Bcl - 2 mRNA in VSMCs among 5 groups of rats(P ﹥0. 05). There was significant difference in VSMCs apoptosis rate among 5 groups of rats(P ﹤ 0. 05). The VSMCs apoptosis rate in high phosphorus group and in vitamin K2 group 1 was significantly higher than that in control group,the VSMCs apoptosis rate in vitamin K2 group 1,in vitamin K2 group 2 and in vitamin K2 group 3 was significantly lower than that in high phosphorus group,respectively(P ﹤ 0. 05). Conclusion Vitamin K2 can inhibit high - phosphorus - induced calcification of rat VSMCs, may alleviate VSMCs apoptosis by increasing Gas6 / Axl expression,the mechanism is probably that vitamin K2 may alleviate VSMCs apoptosis by increasing Gas6 / Axl expression.关键词
肌细胞,平滑肌/维生素 K2/血管钙化/细胞凋亡/大鼠Key words
Myocytes,smooth muscle/Vitamin K2/Vascular calcification/Apoptosis/Rats分类
医药卫生引用本文复制引用
白亚玲,徐金升,张睦清,张胜雷,张俊霞,崔立文,张慧然..细胞凋亡在维生素 K2抑制高磷诱导的大鼠血管平滑肌细胞钙化中的作用[J].中国全科医学,2015,(3):278-282,5.基金项目
河北省自然科学基金资助项目 （）
