中国全科医学Issue(3)：294-299,6.DOI:10.3969/j.issn.1007-9572.2015.03.013
湿润暴露疗法/湿润烧伤膏治疗慢性难愈合创面的超微病理及丝裂原活化蛋白激酶激酶和c - myc mRNA 表达的机制研究
Ultrastructural Pathology and the Mechanism ot Expression ot MAPKK mRNA & c - myc mRNA ot Chronic Retractory Wound Treated by MEBT/MEBO
摘要
Abstract
Objective To study the ultrastructural pathology and the mechanism of expression of MAPKK mRNA & c- myc mRNA of chronic refractory ulcer wound treated by MEBT/ MEBO. Methods A total of 80 SPF male SD rats were randomly divided into four groups by random number table method:blank control group,model group,MEBO ointment group and new healing lotion group,20 rats in each group. The full - thickness skin defect open wound was established among rats in blank control group,full - thickness skin refractory ulcer wound was established among rats in model group,MEBO ointment group and new healing lotion group. After the establishment of wound,rats in blank control group were not managed,rats in model group were dressed with normal saline,rats in MEBO ointment group were dressed with MEBO,rats in new healing lotion group were sprayed with new healing lotion. 3,6,12 days after treatment,the cellular ultrastructural structure of the skin wound was observed using TEM microscope. 12 days after treatment,the expression levels of MAPKK mRNA and c - myc mRNA using RT - PCR technology. Results 3,6 and 12 days after treatment,the cellular ultrastructural structure of the skin wound among rats in MEBO ointment group and new healing lotion group gradually improved,morphology and structure of cell organs restored to normal. There were significant differences in expression levels of MAPKK mRNA and c - myc mRNA among four groups( P ﹤ 0. 05). The expression levels of MAPKK mRNA and c - myc mRNA in model group,MEBO ointment group and new healing lotion group were significantly lower than those in blank control group,respectively;while the expression levels of MAPKK mRNA and c - myc mRNA in MEBO ointment group and new healing lotion group were significantly higher than those in model group,respectively(P ﹤0. 05). There was no significant difference in expression levels of MAPKK mRNA and c - myc mRNA between MEBO ointment group and new healing lotion group( P ﹥ 0. 05). Conclusion MEBT/ MEBO can improve cellular pathological ultrastructure,increase the expression levels of MAPKK mRNA and c - myc mRNA,and promote chronic refractory ulcer wound healing.关键词
慢性难愈合创面/湿润暴露疗法/ 湿润烧伤膏/病理学/丝裂原活化蛋白激酶激酶/c - mycKey words
Chronic Refractory Ulcer/MEBT/ MEBO/Pathology/MAPKK/c - myc分类
医药卫生引用本文复制引用
唐乾利,黄欣,王宇,何晓微,王兵,黄许森,李辉,金萌,吕震..湿润暴露疗法/湿润烧伤膏治疗慢性难愈合创面的超微病理及丝裂原活化蛋白激酶激酶和c - myc mRNA 表达的机制研究[J].中国全科医学,2015,(3):294-299,6.基金项目
国家自然科学基金资助项目（81360547）；广西自然科学基金资助项目 （）
