中国全科医学Issue(9)：1023-1028,6.DOI:10.3969/j.issn.1007-9572.2015.09.010
血小板源性生长因子和血小板源性内皮细胞生长因子在内皮细胞和血管平滑肌细胞中的作用研究
Role of PDGF and PD-ECGF in Endothelial Cells and Vascular Smooth Muscle Cells
摘要
Abstract
Objective To investigate the influence of platelet derived growth factor( PDGF)and platelet -derived endothelial cell growth factor( PD-ECGF)on human umbilical endothelial cells EAHY926 and human-aorta vascular smooth muscle cells T/G HA-VSMC by transfecting the plasmids of PDGF and PD-ECGF in vitro and to evaluate the possibility of treating vascular injury by the two kinds of plasmids. Methods pcDNA 3. 1( +)no-load(no-load group),pcDNA 3. 1 ( +) -PDGF-HA( PPH group)and pcDNA 3. 1( +) -PD-ECGF-HA( PPEH group)plasmids were constructed and transfected into EAHY926 and T/G HA-VSMC. MTT method was used to evaluate the influence of endogenous and exogenous PD-ECGF on the proliferation of EAHY926 and T/G HA-VSMC before and after transfection. Cell wound healing assay was used to evaluate the influence of endogenous and exogenous PD-ECGF on the migration speed of EAHY926 and T/G HA-VSMC after transfection. Results MTT method was used to detect the absorbance value of EAHY926 among the three groups,and the differences were statistically significant(F=235. 18,P<0. 001),with the PPH group significantly higher than the no-load group and PPEH group( P <0. 05 ) . The no - load group and PPEH group showed no statistically significant differences(P>0. 05). MTT method was also used to detect the absorbance value of T/G HA-VSMC among the three groups, and the differences were statistically significant(F=82. 89,P<0. 001),with the PPH group significantly higher than the no-load group(P<0. 05),and the PPEH group significantly lower than the no-load group(P<0. 05). MTT method detecting the influence of medium transfected with PPEH on the proliferation of EAHY926 and T/G HA-VSMC induced by PPH. There was no significant difference in the absorbance value of EAHY926 among no-load group,PPH group and PPH+L-PPEH group ( F=512. 89,P=0. 183). There was significant difference in the absorbance value of T/G HA-VSMC among no-load group, PPH group and PPH+L-PPEH group(F=317. 40,P<0. 001),with the PPH group significantly higher than the no-load group and PPH+L-PPEH group ( P <0. 05 ) . Cell wound healing assay demonstrated that EAHY926 and T/G HA-VSMC transfected with PPEH plasmid had a higher migration ability;compared with the no-load group,the average percentage of cell migration distance of EAHY926 in PPEH group was significantly increased〔(56. 1% ±2. 2%)vs. (27. 8% ±3. 4%)〕(t=-15. 08,P < 0. 001 );compared with the no - load group, the average percentage of cell migration distance of T/G HA-VSMC in PPEH group was significantly increased〔(69. 1% ±2. 3%)vs. (43. 6% ±5. 8%)〕(t= -33. 64,P<0. 001). EAHY926 and T/G HA-VSMC cultured in medium transfected with PPEH showed that the migration ability of EAHY926 and T/G HA-VSMC transfected with PPEH processed by exogenous PD-ECGF also increased significantly. Conclusion After transfection of PDGF plasmid,the stimulation of PDGF and/or PD-ECGF on endothelial cells and smooth muscle cells is simulated. It is found that PDGF can significantly up - regulate the proliferation and migration of endothelial cells and smooth muscle cells. PD-ECGF has limited influence on the proliferation of vascular endothelial cells,but can up-regulate the migration speed of vascular endothelial cells and vascular smooth muscle cells. Meanwhile,PD-ECGF can inhibit the proliferation of vascular endothelial cells. The combined application of the two factors can inhibit over-proliferation of smooth muscle cells and promote migration of endothelial cells and smooth muscle cells. The combination may become a new strategy for the recovery of vascular damage and prevention of vascular restenosis after PCI.关键词
血小板源性生长因子/血小板源性内皮细胞生成因子/血管成形术,气囊,冠状动脉/肌细胞,平滑肌/内皮细胞/细胞增殖Key words
Platelet-derived growth factor/Platelet-derived endothelial cell growth factor/Angioplasty,balloon, coronary/Myocytes,smooth muscle/Endothelial cells/Cell proliferation分类
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朱晋坤,毛华,尹扬光,董文,杜峰,鲁玉明,熊宗华,邓梦扬..血小板源性生长因子和血小板源性内皮细胞生长因子在内皮细胞和血管平滑肌细胞中的作用研究[J].中国全科医学,2015,(9):1023-1028,6.基金项目
贵州省科技厅社会发展项目 （）
