中国全科医学Issue(15)：1743-1747,5.DOI:10.3969/j.issn.1007-9572.2015.15.003
毒毛旋花子阿洛糖苷诱导胃腺癌细胞 SGC -7901凋亡作用及机制研究
Apoptosjs of Gastrjc Adenocarcjnoma Cells Type SGC-7901 Induced by Strophallosjde and Its Mechanjsm
摘要
Abstract
Objectjve To explore the apoptosis - inducing effect of Strophalloside on gastric adenocarcinoma cells type SGC - 7901 and its mechanism. Methods The human gastric adenocarcinoma cells type SGC - 7901 were divided into control group,0. 125 μg/ ml group,0. 250 μg/ ml group,0. 500 μg/ ml group,and 1. 000 μg/ ml group. The growth inhibition effect of Strophalloside on gastric adenocarcinoma cells type SGC - 7901 was detected by MTT assay,and the apoptosis was measured by Annexin V - FITC/ PI flow cytometry and Hoechst staining. The changes of mitochondrial membrane potential were examined by JC - 1 kit. The expressions of mitochondrial pathway - related protein cytochrome C,Caspase - 3 and Caspase - 9 were detected by Western blotting analysis. Results According to MTT assay results,there were significant differences in value A and rate of growth inhibition of gastric adenocarcinoma cells type SGC - 7901 among different groups at 24 h and 48 h after treatment, respectively(P < 0. 05);comparison of the above two indicators between groups showed that value A of gastric adenocarcinoma cells type SGC - 7901 decreased sequentially,and rate of growth inhibition increased sequentially(P < 0. 05). By fluorescence microscope,the cell nucleus of control group showed homogeneous blue fluorescence,the cell nucleus of 0. 500 μg/ ml group showed compact bright fluorescence with white color. There was significant difference in total relative mortality of gastric adenocarcinoma cells type SGC - 7901 among different groups( P < 0. 05);comparison of the above indicator between groups showed that total relative mortality of gastric adenocarcinoma cells type SGC - 7901 increased sequentially( P < 0. 05 ). According to results of JC - 1 staining,gastric adenocarcinoma cells type SGC - 7901 showed green fluorescence 12 h after Strophalloside treatment,and fluorescence intensity increased as concentration of Strophalloside rised. According to Western blotting results,cytochrome C was released from mitochondria into cytoplasm C,and Caspase - 3 and Caspase - 9 were activated,shear and activation occurred,the expression level of activated fragments increased. Conclusjon Strophalloside can induce apoptosis of gastric adenocarcinoma cells type SGC - 7901 through the mitochondria - mediated pathway.关键词
胃肿瘤/毒毛旋花子甙类/线粒体/细胞凋亡Key words
Stomach neoplasms/Strophanthins/Mitochondria/Apoptosis分类
医药卫生引用本文复制引用
张雪娇,梅文莉,蔡彩虹,董文化,姜北,黄风迎,戴好富..毒毛旋花子阿洛糖苷诱导胃腺癌细胞 SGC -7901凋亡作用及机制研究[J].中国全科医学,2015,(15):1743-1747,5.基金项目
国家自然科学基金资助项目（30860345）；国家科技支撑计划课题资助项目（2013BAI11B04）；公益性行业（农业）科研专项 （）
